In recent years, there has been a growing interest in obtaining probiotics bacteria from plant origin. This is the case of Lactiplantibacillus pentosus LPG1, a lactic acid bacteria strain isolated from table olive biofilms with proved multifunctional features. In this work, we have sequenced and closed the complete genome of L. pentosus LPG1 using both Illumina and PacBio technologies. Our intention is to carry out a comprehensive bioinformatics analysis and whole-genome annotation for a further complete evaluation of the safety and functionality characteristics of this microorganism. The chromosomic genome had a size of 3,619,252 bp, with a GC content of 46.34%. L. pentosus LPG1 had also two plasmids, designated as pl1LPG1 and pl2LPG1, with lengths of 72,578 and 8,713 bp, respectively. Genome annotation revealed that the sequenced genome consisted of 3,345 coding genes and 89 non-coding sequences (73 tRNA and 16 rRNA genes). Taxonomy was confirmed by Average Nucleotide Identity analysis, which grouped L. pentosus LPG1 with other sequenced L. pentosus genomes. Moreover, pan-genome analysis showed that L. pentosus LPG1 was closely related with the L. pentosus strains IG8, IG9, IG11, and IG12, all them also isolated from table olive biofilms. Resistome analysis reported absence of antibiotic resistance genes, whilst PathogenFinder tool classified the strain as a non-human pathogen. Finally, in silico analysis of L. pentosus LPG1 showed that very of its previously reported technological and probiotic phenotypes had correspondence with presence of functional genes. In light of these results, we can conclude that L. pentosus LPG1 is a safe microorganism with a great potential for its use as starter culture and plant-based probiotic.
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