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RNA was isolated from drought treated (70% and 40% dehydration stress) and untreated Rhus tripartita seedlings and was used to obtain first and second strand of cDNA, then Differential display reverse transcription PCR (DDRT-PCR) was used to compare overall differences in gene expression between water-stressed (70% and 40% dehydration stress) and control plants. Differentially expressed bands were isolated, cloned and sequenced.Semi-quantitative RT-PCR (SqRT-PCR) and Relative quantitative RT-PCR (Real-time RT-PCR) were used to validate differential display results.
Nucleotide
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