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Genome sequencing sample of Lachnospiraceae bacterium NBRC 112778

Identifiers
BioSample: SAMD00112399
Organism
Lachnospiraceae bacterium KM106-2
cellular organisms; Bacteria; Terrabacteria group; Bacillota; Clostridia; Lachnospirales; Lachnospiraceae; unclassified Lachnospiraceae
Package
MIGS: cultured bacteria/archaea, wastewater; version 6.0
Attributes
sample nameLachnospiraceae bacterium NBRC 112778
observed biotic relationshipfree living
collection date2017-01-06
culture collectionNBRC:112778
broad-scale environmental contextfreshwater biome
local-scale environmental contextmethane fermentation tank
environmental mediumwater
estimated size4500000
geographic locationJapan:Fukushima, Date
isolation and growth conditionnot provided
latitude and longitude37.76125 N 140.57864 E
number of replicons1
project nameNew bacteria whole genome sequencing, isolated from methane fermentator
reference for biomaterialnot provided
sample material processingThis bacterium was originally isolated from a mesophilic methanogenic bioreactor digesting sweet potato. Methanogenic sludge was inoculated onto an agar plate of RCM (reinforced clostridial medium) containing Red Starch as an indicator for amylase (grams per liter): yeast extract, 13; peptone, 10; glucose, 5; Red Starch, 1; NaCl, 5; sodium acetate, 3, Cysteine-HCl 0.5 (pH6.8) and anaerobically incubated at 37 degrees C for three days using AnaeroPack pouch. Colonies forming clear halo were isolated and purified by successive dilution and cultivation in the same condition. For pure cultivation, RCM containing 0.1 % normal soluble starch was used.
strainKM106-2
trophic levelheterotroph
locus_tag_prefixlbkm
Description

Keywords: GSC:MIxS;MIGS:6.0

BioProject
PRJDB6766 Lachnospiraceae bacterium KM106-2
Retrieve all samples from this project

Submission
College of Bioresource Sciences, Nihon University; 2019-11-18
Accession:
SAMD00112399
ID:
13323271

BioProject SRA Nucleotide

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