BioSample

Enterobacter cloacae strain Hanford was isolated from an enriched consortia resulting from lactate amended bioreactors that were inoculated with groundwater from a well located in the chromium contaminated 100H area at Hanford, WA, USA . The enriched consortia were then serially diluted and plated for colony isolation. All work was conducted anaerobically. Picked colonies were grown in the same liquid medium as used in the bioreactors. Use of fructose rather then than lactatose (each 30 mM) as the carbon source resulted in more rapid and more robust growth and this was used in the standard growth medium for the strain