Testing different rRNA removal methods in 4 species of model halophilic archaea - - GEO DataSets

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Testing different rRNA removal methods in 4 species of model halophilic archaea

(Submitter supplied) We tested a number of rRNA removal methods (Illumina RiboZero Plus, NEBNext, NEB Core Depletion Set with custom probes, siTools Panarchaea, siTools RiboPool) on 4 model halophile species: Halobacterium salinarum, Haloferax volcanii, Haloferax meditteranei, Haloarcula hispanica). It was found that methods using custom probes (NEB Core Depletion set with HVO probes, siTools RiboPool with HVO probes) efficiently remove rRNA in species they are targeted to, and that Panarchaea efficiently removes rRNA in all 4 tested species.

Organism:: Haloferax volcanii; Haloferax mediterranei; Haloarcula hispanica; Halobacterium salinarum

Type:: Expression profiling by high throughput sequencing

4 related Platforms
39 Samples

Download data: TXT

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Accession:: GSE200776

ID:: 200200776

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Testing different rRNA removal methods in 4 species of model halophilic archaea
Testing different rRNA removal methods in 4 species of model halophilic archaea
Accession: GSE200776

GEO DataSets
GPL32164[RGSE] (1)
GEO DataSets
ASM2460057v1 - Genome - Assembly - NCBI
ASM2460057v1 - Genome - Assembly - NCBI

Assembly
Halobacterium salinarum DSM 3754 Ga0061114_scaffold00040.40, whole genome shotgu...
Halobacterium salinarum DSM 3754 Ga0061114_scaffold00040.40, whole genome shotgun sequence
gi|1733319783|ref|NZ_VRYN01000040.1 |WGS:NZ_VRYN01|Ga0061114_scaffold00040.40

Nucleotide
Lactobacillus delbrueckii subsp. bulgaricus strain IMAU62101 16S ribosomal RNA g...
Lactobacillus delbrueckii subsp. bulgaricus strain IMAU62101 16S ribosomal RNA gene, partial sequence
gi|516556822|gb|KF149742.1|

Nucleotide

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