GEO DataSets

(Submitter supplied) The interactions between Gram-negative respiratory pathogens and the host environment at the site of infection largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into this ecological transition, we characterized the transcriptional responses of Pseudomonas aeruginosa PA14, Burkholderia thailandensis E264, Klebsiella pneumoniae MGH 78578, and Stenotrophomonas maltophilia K279A exposed to purified pulmonary surfactant (Survanta) through microarrays. more...

Organism:

Stenotrophomonas maltophilia K279a; Pseudomonas aeruginosa PA14; Klebsiella pneumoniae MGH 78578; Burkholderia thailandensis E264

Type:

Expression profiling by array

Platform:

GPL24615

8 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Burkholderia thailandensis is a soil-dwelling bacterium that shares many metabolic pathways with the ecologically similar, but evolutionarily distant, Pseudomonas aeruginosa. Among the diverse nutrients it can utilize is choline, which can be converted into the osmoprotectant glycine betaine and further catabolized as a source of carbon and nitrogen, similar to P. aeruginosa. Orthologs of genes in the choline catabolic pathway in these two bacteria showed distinct differences in gene arrangement as well as an additional orthologous transcriptional regulator in B. more...

Organism:

Burkholderia thailandensis E264

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21888

16 Samples

Download data: XLSX

(Submitter supplied) Bacterial small RNAs (sRNAs) regulate gene expression by base-pairing with downstream target mRNAs to attenuate translation of mRNA into protein at the post-transcriptional level. In response to specific environmental changes, sRNAs can modulate the expression levels of target genes, thus enabling adaptation of cellular physiology. We profiled sRNA expression in the Gram-negative bacteria Burkholderia thailandensis cultured under 54 distinct growth conditions using a Burkholderia-specific microarray that contains probe sets to all intergenic regions (IGRs) greater than 90 bases. more...

Organism:

Burkholderia thailandensis E264

Type:

Expression profiling by array

Platform:

GPL18529

162 Samples

Download data: CEL

(Submitter supplied) see manufacturer's web site

Organism:

Burkholderia thailandensis E264; Stenotrophomonas maltophilia K279a; Pseudomonas aeruginosa PA14; Klebsiella pneumoniae MGH 78578

1 Series

8 Samples

Download data: CDF, CSV, GIN, TXT

(Submitter supplied) A custom Affymetrix microarray was designed using Burkholderia thailandensis E264 gene annotations from Pathema and included 8711 probe sets targeting 5557 protein-coding genes and 2908 intergenic regions (IGRs) longer than 90 bases on the plus strand Protocol: in situ oligonucleotide synthesis (see manufacturer's web site)

Organism:

Burkholderia thailandensis E264

1 Series

162 Samples

Download data: CDF, GFF3, GIN, SIF, TXT