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Links from GEO DataSets

Items: 20

1.

Transcriptome of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization

(Submitter supplied) Transcription profiling of wild type E. coli MG1655, intestine-adapted E. coli MG1655star, and E. coli MG1655 flhD mutant grown on glucose, mannose, and mucus. We previously isolated a spontaneous mutant of E. coli K-12, strain MG1655, following passage through the streptomycin-treated mouse intestine, which has colonization traits superior to the wild-type parent strain (Leatham, et. al., 2005, Infect Immun 73:8039-49) The intestine-adapted strain (E. more...
Organism:
Escherichia coli CFT073; Bacillus anthracis; Bacteroides thetaiotaomicron VPI-5482; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; Escherichia coli O157:H7 str. Sakai; Escherichia coli O157:H7 str. EDL933; Enterococcus faecalis V583; Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by array
Platform:
GPL6702
13 Samples
Download data: CEL, TXT
Series
Accession:
GSE25106
ID:
200025106
2.

Carbon nutrition of pathogenic and commensal E. coli

(Submitter supplied) Transcriptome profiling of E. coli MG1655, E. coli EDL933, E. coli ECOR26, and E. coli Nissle 1917 grown on mouse cecal mucus as carbon source The carbon sources that support growth of pathogenic E. coli O157:H7 in the mammalian intestine have not previously been investigated. In vivo, pathogenic E. coli EDL933 primarily grows as dispersed single cells within the mucus layer that overlies the mouse cecal epithelium. more...
Organism:
Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; Bacillus anthracis; Escherichia coli CFT073; Bacteroides thetaiotaomicron VPI-5482; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655; Escherichia coli; Escherichia coli O157:H7 str. EDL933; Enterococcus faecalis V583
Type:
Expression profiling by array
Platform:
GPL6702
16 Samples
Download data: CEL, TXT
Series
Accession:
GSE11254
ID:
200011254
3.

RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens

(Submitter supplied) Here, we investigated the impact of Stx2 phage carriage on Escherichia coli (E. coli) K-12 MG1655 host gene expression. Using quantitative RNA-seq analysis, we compared the transcriptome of naïve MG1655 and the lysogens carrying the Stx2 phage of the 2011 E. coli O104:H4 outbreak strain or of the E. coli O157:H7 strain PA8, which share high degree of sequence similarity.
Organism:
Escherichia coli K-12
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26204
9 Samples
Download data: CSV
Series
Accession:
GSE126710
ID:
200126710
4.

Nitrogen nutrition of E. coli in the mammalian intestine

(Submitter supplied) Our focus of this study is to determine the differential expression of genes related to uptake and degradation of nitrogenous compounds in E. coli colonizing the mouse intestine as compared to E. coli grown in minimal medium in vitro. We colonized CD-1 male mice with E. coli MG1655 StrR wild type and extracted the total RNA from mouse cecal mucus (GSE217743). We also colonized CD-1 male mice with E. more...
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26592
10 Samples
Download data: WIG
Series
Accession:
GSE241658
ID:
200241658
5.

OmpC and bile tolerance are important for Escherichia coli colonization of the mammalian intestine

(Submitter supplied) Our focus of this study is to determine the gene expression changes in E. coli colonizing the mouse intestine as compared to E. coli grown in minimal medium in vitro. We colonized CD-1 male mice with E. coli MG1655 StrR wild type and extracted the total RNA from mouse cecal mucus. We also extracted E. coli MG1655 StrR RNA from in vitro culture and sequenced and compared to determine the genes important for E. more...
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by high throughput sequencing
Platform:
GPL26592
5 Samples
Download data: WIG
Series
Accession:
GSE217743
ID:
200217743
6.

Transcriptome analysis of wild type E. coli (K-12 MG1655) comparing to mutant E. coli strain (ECOM4) under aerobic and anaerobic conditions

(Submitter supplied) Cytochrome oxydases and quinol monooxygenase were removed from the E. coli genome resulting in oxygen-independent physiology We used microarray profiling to identify perturbed metabolic or regulatory functionality resulting in the inability to undergo aerobic-anaerobic shift.
Organism:
Escherichia coli; Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by array
Platform:
GPL3154
12 Samples
Download data: CEL
Series
Accession:
GSE21839
ID:
200021839
7.

Expression Profiling E. Coli Evolved on Glucose Minimal Media

(Submitter supplied) mRNA sequencing was performed on E. coli evolved at 37C on M9 Minimal Media with Glucose
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17439
11 Samples
Download data: CSV
Series
Accession:
GSE61327
ID:
200061327
8.

Effect of simulated microgravity on E. coli K12 MG1655 growth and gene expression

(Submitter supplied) This study demonstrates simulated microgravity effects on E. coli K 12 MG1655 when grown on LB medium supplemented with glycerol. The results imply that E. coli readily reprograms itself to combat the multiple stresses imposed due to microgravity. Under these conditions it survives by upregulating oxidative stress protecting genes and simultaneously down regulating the membrane transporters and synthases to maintain cell homeostasis.
Organism:
Escherichia coli; Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by array
Platform:
GPL3154
8 Samples
Download data: CEL, CHP
Series
Accession:
GSE40648
ID:
200040648
9.

Caveat Mutator

(Submitter supplied) The use of whole genome microarrays for monitoring mutagenised or otherwise engineered genetic derivatives is a potentially powerful tool for checking genomic integrity. Using comparative genomic hybridization of a number of unrelated, directed deletion mutants in Escherichia coli K-12 MG1655 we identified unintended secondary genomic deletions in the flhDC region in deltafnr, deltacrp, and deltacreB mutants. more...
Organism:
Escherichia coli
Type:
Expression profiling by array; Genome variation profiling by array
Platform:
GPL3051
5 Samples
Download data
Series
Accession:
GSE7695
ID:
200007695
10.

A reassesment of the FNR regulon and the effects of nitrate,nitrite, narXL and narQP

(Submitter supplied) The fumarate and nitrate reductase regulator protein, FNR, is a global transcription factor that regulates major biochemical changes as Escherichia coli adapts from aerobic to anaerobic growth. The ability of an fnr mutant to grow anaerobically in the presence of trimethylamine-N-oxide (TMAO) as the terminal electron acceptor was exploited in microarray experiments designed to determine a minimum number of Escherichia coli K-12 MG1655 operons that are regulated directly by FNR. more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platforms:
GPL3051 GPL1246
55 Samples
Download data
Series
Accession:
GSE3591
ID:
200003591
11.

Genome-scale reconstruction of the PurR regulon reveals its role in the adenine stimulon of Escherichia coli K-12 MG1655

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Escherichia coli str. K-12 substr. MG1655; Escherichia coli
Type:
Expression profiling by array; Genome binding/occupancy profiling by genome tiling array
Platforms:
GPL3154 GPL8387
12 Samples
Download data: CEL, PAIR
Series
Accession:
GSE26591
ID:
200026591
12.

ChIP-chip of E. coli K-12 MG1655 with antibody against PurR-8myc under various conditions.

(Submitter supplied) We integrated transcription factor binding regions and mRNA transcript abundance to elucidate the PurR regulon experimentally. To measure transcription factor binding at a genome scale, we employed a ChIP-chip method to derivative strains of E. coli K-12 MG1655 harboring PurR-8myc under various conditions.
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Genome binding/occupancy profiling by genome tiling array
Platform:
GPL8387
4 Samples
Download data: PAIR
Series
Accession:
GSE26589
ID:
200026589
13.

Transcriptome analysis of E. coli MG1655

(Submitter supplied) Expression profiling of wild type and purR deletion strains of E. coli K-12 MG1655 under both M9 minimal media and addition of adenine.
Organism:
Escherichia coli; Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by array
Platform:
GPL3154
8 Samples
Download data: CEL
Series
Accession:
GSE26588
ID:
200026588
14.

Effect of the global regulators RpoS and cyclic-AMP/CRP on the transcriptome of Escherichia coli K12 during carbon- and energy-limited growth

(Submitter supplied) The faecal indicator bacterium Escherichia coli K12 was used to study the effects of the global regulators RpoS and cAMP at the transcription level using microarray technology during short-term (physiological) adaptation to slow growth under limited nutrient supply. Effects due to the absence of one global regulator were assessed by comparing the mRNA levels isolated from rpoS or cya mutants under glucose-limited continuous culture at a dilution rate of 0.3 h-1 for the rpoS mutant or 0.16 h-1 for the cya mutant with those from wt E. more...
Organism:
Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL534
6 Samples
Download data
Series
Accession:
GSE25982
ID:
200025982
15.

The expression profile of Escherichia coli K-12 in response to minimal, optimal and excess copper concentrations

(Submitter supplied) The gene expression profile of E. coli K-12 MG1655 grown in minimal medium supplemented with elevated copper concentrations (as copper-glycine) has been analysed using whole genome oligonucleotide microarrays. “Pool” RNA was isolated from five independently grown 50ml DMA cultures of E. coli K-12 MG1655. “Test” RNA was isolated from three independently grown 50ml DMA cultures of E. coli K-12 MG1655, for each growth condition (no added CuSO4, or supplemented with either 0.75mM or 2mM copper glycine). more...
Organism:
Escherichia coli
Type:
Expression profiling by array
Platform:
GPL1246
9 Samples
Download data
Series
Accession:
GSE1780
ID:
200001780
16.

luxS-Dependent Gene Regulation in Escherichia coli K-12 Revealed by Genomic Expression Profiling (II)

(Submitter supplied) The bacterial quorum-sensing autoinducer 2 (AI-2) has received intense interest because the gene for its synthase, luxS, is common among a large number of bacterial species. We have identified luxS-controlled genes in Escherichia coli under two different growth conditions using DNA microarrays. Twenty-three genes were affected by luxS deletion in the presence of glucose, and 63 genes were influenced by luxS deletion in the absence of glucose. more...
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL199
4 Samples
Download data: CEL, CHP
Series
Accession:
GSE9587
ID:
200009587
17.

luxS-Dependent Gene Regulation in Escherichia coli K-12 Revealed by Genomic Expression Profiling

(Submitter supplied) The bacterial quorum-sensing autoinducer 2 (AI-2) has received intense interest because the gene for its synthase, luxS, is common among a large number of bacterial species. We have identified luxS-controlled genes in Escherichia coli under two different growth conditions using DNA microarrays. Twenty-three genes were affected by luxS deletion in the presence of glucose, and 63 genes were influenced by luxS deletion in the absence of glucose. more...
Organism:
Escherichia coli; Escherichia coli K-12
Type:
Expression profiling by array
Platform:
GPL199
4 Samples
Download data: CEL, CHP
Series
Accession:
GSE9582
ID:
200009582
18.

Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing
Platform:
GPL17439
6 Samples
Download data: GFF
Series
Accession:
GSE88981
ID:
200088981
19.

Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [RNA-seq]

(Submitter supplied) A transcription factor (TF), OmpR, plays a critical role in transcriptional regulation of the defense system for osmotic stress in bacteria. However, its full genome-wide regulatory potential is unknown. Here, we perform a genome-scale reconstruction of the OmpR regulon in Escherichia coli K-12 MG1655. Integrative data analysis reveals that a total of 37 genes in 24 transcription units (TUs) belong to OmpR regulon. more...
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17439
4 Samples
Download data: CSV
Series
Accession:
GSE88980
ID:
200088980
20.

Revealing the genome-scale transcriptional regulatory landscape of OmpR highlights its expanded regulatory roles and unexpected importance of narU under osmotic stress in Escherichia coli K-12 MG1655 [ChIP-exo]

(Submitter supplied) A transcription factor (TF), OmpR, plays a critical role in transcriptional regulation of the defense system for osmotic stress in bacteria. However, its full genome-wide regulatory potential is unknown. Here, we perform a genome-scale reconstruction of the OmpR regulon in Escherichia coli K-12 MG1655. Integrative data analysis reveals that a total of 37 genes in 24 transcription units (TUs) belong to OmpR regulon. more...
Organism:
Escherichia coli str. K-12 substr. MG1655
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL17439
2 Samples
Download data: GFF
Series
Accession:
GSE88979
ID:
200088979
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