GEO DataSets

(Submitter supplied) RIP-seq analysis to identify CjCas9 bound RNAs using co-immunoprecipitation and sequencing in CG84-21.

Organism:

Campylobacter jejuni subsp. jejuni CG8421

Type:

Other

Platform:

GPL29023

6 Samples

Download data: WIG

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Campylobacter jejuni subsp. jejuni CG8421

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL29024 GPL29023

10 Samples

Download data: WIG

(Submitter supplied) Differential RNA sequencing using terminator exonuclease (TEX) to identify processed versus unprocessed transcripts in Campylobacter jejuni strain CG84-21

Organism:

Campylobacter jejuni subsp. jejuni CG8421

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29024

4 Samples

Download data: WIG

(Submitter supplied) This study investigates the RNA targets and cleavage sites of endogenous Cas9 in the food-borne pathogen Campylobacter jejuni. Direct RNA binding targets of Cas9 in C. jejuni strain NCTC11168 were determined using RIP-seq. The Cleavage sites were then predicted in the RNA targets by comparing total transcriptome data from WT and deletion (cas9, crRNA3, tracrRNA, CRISPR-tracrRNA) strains. PAMs for the CjeCas9 were enriched using the PAM-SCANR platform, which operates through a GFP reporter gene. more...

Organism:

Escherichia coli K-12; Campylobacter jejuni

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL19974 GPL19168

13 Samples

Download data: TXT, WIG, XLSX

(Submitter supplied) Circular RNAs (circRNAs) are widely expressed, but their functions remain largely unknown. To study circRNAs in a high-throughput manner, short hairpin RNA (shRNA) screens1 have recently been used to deplete circRNAs by targeting their unique back-splicing junction (BSJ) sites. Here, we report frequent discrepancies between shRNA-mediated circRNA knockdown efficiency and the corresponding biological effect, raising pressing concerns about the robustness of shRNA screening for circRNA functional characterization. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing; Other

4 related Platforms

22 Samples

Download data: TXT