GEO DataSets

(Submitter supplied) Sortase-assembled pili contribute to virulence in many Gram-positive bacteria. In Enterococcus faecalis, the endocarditis and biofilm-associated pilus (Ebp) is polymerized on the membrane by sortase C (SrtC) and attached to the cell wall by sortase A (SrtA). In the absence of SrtA, polymerized pili remain anchored to the membrane (i.e. off-pathway). Here we show that the high temperature requirement A (HtrA) bifunctional chaperone/protease of E. more...

Organism:

Enterococcus faecalis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18799 GPL24867

33 Samples

Download data: XLSX

(Submitter supplied) Enterococcus faecalis is a commensal bacterium in the gastrointestinal tract (GIT) of humans and other organisms. Outside of the GIT, E. faecalis can cause infections in root canals, wounds, surgical sites, the urinary tract, and on heart valves. Many of these infections can be polymicrobial, and a variety of toxins and nutritional signals influence interactions between E. faecalis and other microbes. more...

Organism:

Enterococcus faecalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31169

8 Samples

Download data: TXT

(Submitter supplied) Cas9 is a transcriptional autoregulator in many strains of S. pyogenes. A natural single guide RNA (tracr-L) directs Cas9 to bind to and repress its promoter. In this study, we aimed to determine whether tracr-L, crRNAs, or other noncanonical guide RNAs direct Cas9 to bind to other regions of the genome and potentially regulate the expression of additional genes.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28678

18 Samples

Download data: SF

(Submitter supplied) Cas9 is a transcriptional autoregulator in many strains of S. pyogenes. A natural single guide RNA (tracr-L) directs Cas9 to bind to and repress its promoter. In this study, we aimed to determine whether tracr-L, crRNAs, or other noncanonical guide RNAs direct Cas9 to bind to other regions of the genome and potentially regulate the expression of additional genes.

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19982

12 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) Extracellular vesicles (EVs) are released by most cell types and are implicated in several biological and pathological processes, including multiple sclerosis (MS). In this study we performed RNA sequencing to analyze the diversity of microorganisms by assignment of reads using different taxa profilers. To diminish the risk of false positive biases derived from sample handling, we performed a similar analysis on EVs derived from known cultured bacterial species, as well as artificially-generated samples. more...

Organism:

Bifidobacterium animalis subsp. lactis; Lactobacillus acidophilus; Homo sapiens; synthetic construct

Type:

Expression profiling by high throughput sequencing

4 related Platforms

15 Samples

Download data: CSV

(Submitter supplied) In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that controls different stress-induced phenotypes. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the CSP-induced stationary phase cells. more...

Organism:

Streptococcus mutans UA159

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33790

6 Samples

Download data: TXT

(Submitter supplied) Streptococcus agalactiae is among the few pathogens that have not developed resistance to ß-lactam antibiotics despite decades of clinical use. The molecular basis of this long-lasting susceptibility has not been investigated, and it is uncertain whether specific mechanisms constraint the emergence of resistance. In this study, we first report a conserved role of the signaling nucleotide cyclic-di-AMP in the sensitivity of S. more...

Organism:

Streptococcus agalactiae; Streptococcus agalactiae NEM316

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29157 GPL34325

18 Samples

Download data: TXT

(Submitter supplied) We have conducted RNA-seq analysis on Group B Streptococcus (GBS) when interacting with human stem-cell (hSC) derived BECs. The study contains a control group for GBS and a control group for BECs each with three biological replicates. The last group contains GBS infection BECs and also has three replicates.

Organism:

Streptococcus sp. 'group B'; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18573 GPL31998 GPL31999

9 Samples

Download data: CSV

(Submitter supplied) This study investigates the regulons of 11 pneumococcal response regulators (RRs) by RNA-sequencing of RR-overexpression mutants.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28680

36 Samples

Download data: CSV

(Submitter supplied) The tRNA gene expression change was evaluated by whole genomic microarray chip in Streptococcus oligofermentans challenged by H2O2.

Organism:

Streptococcus cristatus

Type:

Non-coding RNA profiling by array

Platform:

GPL29985

4 Samples

Download data: TXT

(Submitter supplied) Each bacterial species has specific regulatory systems to control physiology, adaptation, and host interactions. One challenge posed by this diversity is to define the evolving gene regulatory networks. This study aims to characterise two-component systems (TCS) in Streptococcus gagalactiae, the main cause of neonatal meningitis. Here we demonstrate signal-independent activation of signalling pathways by systematically targeting the conserved mechanism of phosphatase activity of the histidine kinases of the two main TCS families. more...

Organism:

Streptococcus agalactiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29157

48 Samples

Download data: XLS

(Submitter supplied) The goal was to identify genes regulated by YvcL (EF0768) by assessing changes in the yvcL deletion mutant relative to the isogenic wild-type. Wild-type E. faecalis OG1 and the mutant lacking yvcL were cultured in Mueller-Hinton broth to exponential phase. Total RNA was purified, depleted for rRNA, and subjected to preparation of mRNA libraries.

Organism:

Enterococcus faecalis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28666

8 Samples

Download data: TXT

(Submitter supplied) Streptococcus pneumoniae (Spn), a Gram-positive bacterium, poses a significant threat to human health, causing mild respiratory infections to severe invasive conditions. Despite availability of vaccines, challenges persist due to serotype replacement and antibiotic resistance, emphasizing the need for alternative therapeutic strategies. This study explores the intriguing role of polyamines, ubiquitous, small organic cations, in modulating virulence factors, especially the capsule, a crucial determinant of Spn's pathogenicity. more...

Organism:

Streptococcus pneumoniae D39

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34053

12 Samples

Download data: CSV

(Submitter supplied) Time-series ribosome profiling experiment. It consists of mock and sk1 bacteriophage infected samples of Lactococcus Lactis at three different timepoints; 2,5 and 15 minutes post infection

Organism:

Lactococcus lactis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23778

16 Samples

Download data: TSV, XLS

(Submitter supplied) We compared differential gene expression in ST399/EMM-77, invasive ST28/EMM-1 and non-invasive ST28/EMM-1, 4 isolates in each group.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

12 Samples

Download data: CSV

(Submitter supplied) Lacticaseibacillus rhamnosus Lcr35 is a well-known bacterial strain whose efficiency in preventing recurrent vulvovaginal candidiasis has been largely demonstrated in clinical trials. The presence of sodium thiosulfate (STS) has been shown to enhance its ability to inhibit the growth of C. albicans strains. In this study, we confirmed that Lcr35 has a fungicidal effect not only on the planktonic form of C. more...

Organism:

Lacticaseibacillus rhamnosus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33752

9 Samples

Download data: TXT

(Submitter supplied) Recent RNA-seq studies have given us a deeper insight into the cariogenic impact of carbohydrate sources in the bacterium Streptococcus mutans, the principal etiological agent of human dental caries. The process of dental caries development is given by the ability of this bacterium to ferment some carbohydrates up to organic acids contributing to the pH decrease in the oral cavity and the demineralization of the enamel. more...

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

15 Samples

Download data: XLSX

(Submitter supplied) There had a total number of 804 genes with transcript difference, including 482 up-regulated genes and 322 down-regulated genes. Enrichment analysis of those 804 different transcript gene sets was performed to explore the biological effects and the corresponding pathways that are significantly associated on GO and KEGG (Padj <0.05 as the threshold for significant enrichment). In the GO functional enrichment analysis, all differential genes were significantly enriched into 65 GO Terms, among them there had 50 GO terms belonging to BP (Biological Process). more...

Organism:

Enterococcus durans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30933

12 Samples

Download data: TXT

(Submitter supplied) The gene expression of S. pneumoniae cultured in medium with different concentration of phosphate were detected, aiming to analyze the role of reduced extracellular phosphate in virluence regulation of S. pneumoniae.

Organism:

Streptococcus pneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23152

2 Samples

Download data: CSV, XLS

(Submitter supplied) NIP and NrpR forms a pair for the transcriptional regulator in Streptococcus salivarius K12 (SAL). To explore the target genes regulated by the NIP-NrpR pair in Streptococcus salivarius K12 (SAL), we generated mutants in which the NIP or NrpR gene has been knocked down through complement recombination. Comparative transcriptome profiling of WT, ▲nrpR, and nip* mutant strains revealed that the sar BGC is the major regulatory target controlled by the NIP signaling pathway in SAL.

Organism:

Streptococcus salivarius K12

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33931

9 Samples

Download data: XLSX