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Biochemical EC50 Assay from US Patent US20240050576: "METHODS AND COMPOUNDS FOR THE TREATMENT OF GENETIC DISEASE"
Assay data:51 Active, 61 Activity ≤ 1 µM, 61 Tested
SummaryRelated BioAssays by Target
Inhibition of human GAPDH
Assay data:1 Tested
Irreversible inhibition of GAPDH (unknown origin) assessed as ratio of Kinact/Ki
Assay data:4 Tested
Inhibition of GAPDH (unknown origin) measured after 1 hr
Assay data:2 Tested
Inhibition of rabbit muscle GAPDH at 0.5 mM incubated for 24 hrs relative to control
Irreversible inhibition of GAPDH (unknown origin) assessed as ratio of Kinact/Ki at slow phase
Irreversible inhibition of GAPDH (unknown origin) assessed as ratio of Kinact/Ki at fast phase
cytomteric bead array (CBA) assay from US Patent US9694015: "Methods for the treatment of locally advanced breast cancer"
Assay data:9 Active, 5 Activity ≤ 1 nM, 9 Activity ≤ 1 µM, 10 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Inhibition of GAPDH activity (unknown origin) after 1 hr
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to GAPDH in human A549 cells by LC-MS/MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Covalent binding affinity to GAPDH (unknown origin) by Western blot analysis
Covalent binding affinity to GADPH in human HCT116 cell lysates at 30 uM incubated for 8 hrs by Western blot analysis
SummaryCompounds, ActiveRelated BioAssays by Target
Binding affinity to GADPH in human HCT116 cells at 50 uM incubated for 8 hrs by LC-MS/MS analysis
Binding affinity to GAPDH in human HeLa cell lysates assessed as cysteine-compound adduct formation at 50 uM preincubated for 22 mins followed by IAA-probe addition and measured after 20 mins by mass spectrometric method
Binding affinity to glyceraldehyde-3-phosphate dehydrogenase in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis
Effect on dimer to tetramer equilibrium of recombinant N-terminal His-tagged human GAPDH expressed in Escherichia coli BL21 codonPlus (DE3)-RIL cells at 50 uM after 4 hrs by gel filtration method
Binding affinity to recombinant N-terminal His-tagged human GAPDH expressed in Escherichia coli BL21 codonPlus (DE3)-RIL cells assessed as enzyme Vmax at 50 uM after 4 hrs by Michaelis-Menten plot analysis (Rvb = 0.069 +/- 0.003 mM/min)
Binding affinity to recombinant N-terminal His-tagged human GAPDH expressed in Escherichia coli BL21 codonPlus (DE3)-RIL cells assessed as enzyme Km at 50 uM after 4 hrs by Michaelis-Menten plot analysis (Rvb = 340+/- 5 uM)
Binding affinity to recombinant N-terminal His-tagged human GAPDH expressed in Escherichia coli BL21 codonPlus (DE3)-RIL cells assessed as remaining cysteine pKa at 300 uM after 4 hrs (Rvb = 6)
Binding affinity to recombinant N-terminal His-tagged human GAPDH expressed in Escherichia coli BL21 codonPlus (DE3)-RIL cells assessed as decrease in reactive cysteine content at 50 uM after 4 hrs relative to control
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