Abstract
A polyphasic taxonomic study involving DNA-DNA hybridization, whole-cell protein electrophoresis, and 16S ribosomal DNA sequence analysis revealed that a group of Burkholderia cepacia-like organisms isolated from the rhizosphere or tissues of maize, wheat, and lupine belong to B. cepacia genomovar III, a genomic species associated with "cepacia syndrome" in cystic fibrosis patients. The present study also revealed considerable protein electrophoretic heterogeneity within this species and demonstrated that the B. cepacia complex consists of two independent phylogenetic lineages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / analysis
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Burkholderia cepacia / chemistry
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Burkholderia cepacia / classification*
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Burkholderia cepacia / genetics
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Burkholderia cepacia / isolation & purification
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Crops, Agricultural / microbiology*
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DNA, Ribosomal / analysis
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Electrophoresis, Polyacrylamide Gel
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Molecular Sequence Data
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Nucleic Acid Hybridization / methods
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RNA, Ribosomal, 16S / genetics
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Sequence Analysis, DNA
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Triticum / microbiology
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Zea mays / microbiology
Substances
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Bacterial Proteins
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DNA, Ribosomal
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RNA, Ribosomal, 16S
Associated data
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GENBANK/AF311969
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GENBANK/AF311970
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GENBANK/AF311971
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GENBANK/AF311972