Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland

Luciana Albuquerque; Magdalena Kowalewicz-Kulbat; Dominika Drzewiecka; Paweł Stączek; Giuseppe d'Auria; Ramon Rosselló-Móra; Milton S da Costa

doi:10.1016/j.syapm.2015.12.004

Halorhabdus rudnickae sp. nov., a halophilic archaeon isolated from a salt mine borehole in Poland

Syst Appl Microbiol. 2016 Mar;39(2):100-5. doi: 10.1016/j.syapm.2015.12.004. Epub 2015 Dec 18.

Authors

Luciana Albuquerque¹, Magdalena Kowalewicz-Kulbat², Dominika Drzewiecka³, Paweł Stączek⁴, Giuseppe d'Auria⁵, Ramon Rosselló-Móra⁶, Milton S da Costa⁷

Affiliations

¹ Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal.
² Department of Immunology and Infectious Biology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
³ Department of General Microbiology, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
⁴ Department of Genetics of Microorganisms, Institute of Microbiology, Biotechnology and Immunology, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland.
⁵ Área de Genómica y Salud, Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunidad Valenciana (FISABIO-Salud Pública), Avenida de Cataluña 21, 46020 Valencia, Spain; Instituto Cavanilles de Biodiversidad y Biología Evolutiva, Universitat de València, C/Catedrático José Beltrán 2, 46980 Valencia, Paterna, Spain.
⁶ Marine Microbiology Group, Institut Mediterrani d'Estudis Avançats (CSIC-UIB), E-07190 Esporles, Illes Balears, Spain.
⁷ Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal; Microbiology Unit, BIOCANT Biotechnological Park, 3060-197 Cantanhede, Portugal. Electronic address: milton@ci.uc.pt.

PMID: 26749115
DOI: 10.1016/j.syapm.2015.12.004

Abstract

Two halophilic archaea, designated strains WSM-64(T) and WSM-66, were isolated from a sample taken from a borehole in the currently unexploited Barycz mining area belonging to the "Wieliczka" Salt Mine Company, in Poland. Strains are red pigmented and form non-motile cocci that stain Gram-negative. Strains WSM-64(T) and WSM-66 showed optimum growth at 40 °C, in 20% NaCl and at pH 6.5-7.5. The strains were facultative anaerobes. The major polar lipids of the two strains were phosphatidylglycerol (PG2), phosphatidylglycerol phosphate methyl ester (PGP-Me) and sulfated diglycosyl diether (S-DGD). Menaquinone MK-8 was the major respiratory quinone. The DNA G+C content of strain WSM-64(T) was 61.2 mol% by HPLC method; 61.0 mol% by genome sequencing. Analysis of the almost complete 16S rRNA gene sequence indicated that the strains WSM-64(T) and WSM-66 (99.7% identity) represented a member of the genus Halorhabdus in the family Halobacteriaceae. Both strains formed a distinct cluster and were most closely related to Halorhabdus tiamatea SARL4B(T) and Halorhabdus utahensis AX-2(T) (DSM 12940(T)) (95.4% and 95.6%, respectively). ANI values of WSM-64(T) with the closest relative type strains were <78.5%. Based on 16S rRNA gene sequence and whole genome analyses, physiological and biochemical characteristics we describe a new species represented by strain WSM-64(T) (=DSM 29498(T) =CECT 8673(T)) for which we propose the name Halorhabdus rudnickae sp. nov.

Keywords: Haloarchaea; Halorhabdus; Halorhabdus rudnickae sp. nov.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques
Geologic Sediments / microbiology*
Halobacteriaceae / classification*
Halobacteriaceae / isolation & purification
Poland