Abstract
In this study, the 16S-23S and 23S-5S rRNA intergenic spacer region sequences of Propionibacterium acidipropionici, P. freudenreichii ssp. freudenreichii and ssp. shermanii, P. jensenii and P. thoenii were determined. The sequences were shown to vary greatly between the species. Specific primer pairs were derived from the 16S-23S rRNA spacer sequences and used for the identification of the species by PCR.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA, Ribosomal Spacer
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Dairy Products / microbiology*
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Gene Amplification
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Molecular Sequence Data
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Phylogeny
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Propionibacterium / classification
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Propionibacterium / genetics*
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RNA, Ribosomal, 16S / genetics*
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RNA, Ribosomal, 23S / genetics*
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RNA, Ribosomal, 5S / genetics*
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Species Specificity
Substances
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DNA, Ribosomal Spacer
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RNA, Ribosomal, 16S
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RNA, Ribosomal, 23S
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RNA, Ribosomal, 5S
Associated data
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GENBANK/AF218431
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GENBANK/AF218432
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GENBANK/AF218433
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GENBANK/AF218434
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GENBANK/AF218435
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GENBANK/AF280071
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GENBANK/AF280072
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GENBANK/AF280073
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GENBANK/AF280074
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GENBANK/AF280075