Characterization of a Novel Putative Xer-Dependent Integrative Mobile Element Carrying the bla(NMC-A) Carbapenemase Gene, Inserted into the Chromosome of Members of the Enterobacter cloacae Complex

Antimicrob Agents Chemother. 2015 Oct;59(10):6620-4. doi: 10.1128/AAC.01452-15. Epub 2015 Jul 27.

Abstract

An Enterobacter ludwigii strain was isolated during routine screening of a Japanese patient for carriage of carbapenem-resistant Enterobacteriaceae. PCR analysis revealed the blaNMC-A carbapenemase gene. Whole-genome sequencing revealed that blaNMC-A was inserted in the chromosome and associated with a novel 29.1-kb putative Xer-dependent integrative mobile element, named EludIMEX-1. Bioinformatic analysis identified similar elements in the genomes of an Enterobacter asburiae strain and of other Enterobacter cloacae complex strains, confirming the mobile nature of this element.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Chromosomes, Bacterial / genetics*
  • Enterobacter cloacae / genetics*
  • Genome, Bacterial / genetics*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase

Associated data

  • GENBANK/KR919803
  • GENBANK/LGIV00000000