Multilocus sequence typing and pulsed-field gel electrophoresis analysis of Oenococcus oeni from different wine-producing regions of China

Tao Wang; Hua Li; Hua Wang; Jing Su

doi:10.1016/j.ijfoodmicro.2015.01.006

Multilocus sequence typing and pulsed-field gel electrophoresis analysis of Oenococcus oeni from different wine-producing regions of China

Int J Food Microbiol. 2015 Apr 16:199:47-53. doi: 10.1016/j.ijfoodmicro.2015.01.006. Epub 2015 Jan 12.

Authors

Tao Wang¹, Hua Li², Hua Wang¹, Jing Su¹

Affiliations

¹ College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China.
² College of Enology, Northwest A&F University, Yangling, Shaanxi 712100, China. Electronic address: lihuawine@nwsuaf.edu.cn.

PMID: 25625911
DOI: 10.1016/j.ijfoodmicro.2015.01.006

Abstract

The present study established a typing method with NotI-based pulsed-field gel electrophoresis (PFGE) and stress response gene schemed multilocus sequence typing (MLST) for 55 Oenococcus oeni strains isolated from six individual regions in China and two model strains PSU-1 (CP000411) and ATCC BAA-1163 (AAUV00000000). Seven stress response genes, cfa, clpL, clpP, ctsR, mleA, mleP and omrA, were selected for MLST testing, and positive selective pressure was detected for these genes. Furthermore, both methods separated the strains into two clusters. The PFGE clusters are correlated with the region, whereas the sequence types (STs) formed by the MLST confirm the two clusters identified by PFGE. In addition, the population structure was a mixture of evolutionary pathways, and the strains exhibited both clonal and panmictic characteristics.

Keywords: Multilocus sequence typing; Oenococcus oeni; Phylogenetic analysis; Population genetics; Pulsed-field gel electrophoresis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

China
Electrophoresis, Gel, Pulsed-Field*
Food Microbiology*
Multilocus Sequence Typing*
Oenococcus / genetics*
Oenococcus / isolation & purification