show Abstracthide AbstractMulti-drug resistance Enterobactericeae are a global challenge especially in neonates and are a leading cause of death. We employed conventional microbiology whole genome sequencing to characterised neonatal MDR carriage and infection dynamics at neonatal unit in low income setting (The Gambia West Africa). Skin, and peri-anal were collected from neonates at day 0 and weekly until 28 days after birth and recto-vaginal swabs collected from mothers. Whole genome draft assemblies were used to determine antimicrobial resistance genes carried.