Lactobacillus johnsonii isolate from C57BL/6 mouse oral mucosa - SRA

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SRX13486791: Lactobacillus johnsonii isolate from C57BL/6 mouse oral mucosa
1 ILLUMINA (Illumina NovaSeq 6000) run: 3M spots, 911M bases, 432.5Mb downloads

Design: Genomic DNA samples were normalized to 0.2ng/uL in preparation for whole genome shotgun library preparation using the Illumina Nextera XT library Preparation kit following the manufacturers protocol. Libraries were validated for length (450bp average library length; average insert size of 315bp) and adapter dimer removal using the Agilent TapeStation 4200 D5000 High Sensitivity assay then quantified and normalized using the dsDNA High Sensitivity Assay for Qubit 3.0. Sample libraries were prepared for Illumina sequencing by denaturing and diluting the libraries per manufacturers protocol. All samples were combined into one sequencing pool, proportioned according to expected number of reads, and run as one sample pool on the Illumina NovaSeq 6000 SP 300 cycle sequencing kit, version 1.5 chemistry. Target read depth was achieved per sample with paired end 150bp reads.

Submitted by: University of Connecticut Health Center

Study: Lactobacillus johnsonii MT4 genome sequencing and assembly

PRJNA787656 • SRP352129 • All experiments • All runs

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Whole genome sequencing and characterization of Lactobacillus johnsonii strain MT4, isolated from the oral mucosa of C57BL/6 mice (Jackson Laboratories, USA) receiving a sucrose-enriched diet

Sample: Lactobacillus johnsonii isolate from C57BL/6 mouse oral mucosa

SAMN23838460 • SRS11382857 • All experiments • All runs

Organism: Lactobacillus johnsonii

Library:

Name: MT4

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 3M spots, 911M bases, 432.5Mb

Run	# of Spots	# of Bases	Size	Published
SRR17309641	3,026,429	911M	432.5Mb	2022-01-13

ID:: 18678659

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