Genome sequence of Lactobacillus delbrueckii subsp. bulgaricus KW14_3 - SRA

SRX5282215: Genome sequence of Lactobacillus delbrueckii subsp. bulgaricus KW14_3
1 PACBIO_SMRT (PacBio RS II) run: 3.4M spots, 1G bases, 463.6Mb downloads

Design: For Illumina pair-end sequencing of each strain, at least 1g genomic DNA was used for sequencing library construction. Paired-end libraries with insert sizes of ~300bp were prepared following Illuminas standard genomic DNA library preparation procedure. Purified genomic DNA is sheared into smaller fragments with a desired size by Covaris, and blunt ends are generated by using T4 DNA polymerase. After adding an A base to the 3' end of the blunt phosphorylated DNA fragments, adapters are ligated to the ends of the DNA fragments. The desired fragments can be purified through gel-electrophoresis, then selectively enriched and amplified by PCR. The index tag could be introduced into the adapter at the PCR stage as appropriate and we did a library quality test. At last, the qualified Illumina pair-end library would be used for Illumina Miseq sequencing

Submitted by: Beijing Technology and Business University

Study: Genome of microorganisms from Tibet kefir

PRJNA513828 • SRP176647 • All experiments • All runs

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genome of microorganism from Tibet kefir

Sample:

SAMN10782524 • SRS4281966 • All experiments • All runs

Organism: Lactobacillus delbrueckii subsp. bulgaricus

Library:

Name: Lactobacillus delbrueckii subsp. bulgaricus KW14_3

Instrument: PacBio RS II

Strategy: WGS