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SRX7004908: Genome sequencing of Lactobacillus plantarum L10: a bacterial isolated from Brazilian ethanol industrial fermentations
1 ILLUMINA (Illumina HiSeq 2500) run: 3.4M spots, 689.9M bases, 518.4Mb downloads

Design: It was obtained the isolate L10 DNA from a cell pellet after cultivating the bacterium in liquid MRS medium for 10h, 30 C and 50 RPM. We generated one short insert size paired-end library by using the Nextera DNA preparation kit, following the recommendations of the manufacturer. We sequenced the library on HiSeq2500 equipment.
Submitted by: Univesity of Sao Paulo
Study: Bacterial species found as contaminants in Brazilian ethanol industrial fermentations
show Abstracthide Abstract
Brazilian ethanol production industries are not contamination-free environments, and it is common to observe other microorganisms than Saccharomyces cerevisiae. Probably the main origin of these microorganisms is the sugarcane juice itself, since the industrial environment is highly selective and marked by stresses such as high temperatures and sugar concentrations. Theoretically, only organisms adapted to these conditions, with a long history of coevolution with S. cerevisiae yeasts, would be able to tolerate these environments. Lactic Acid Bacteria (LAB) and Acetic Acid Bacteria (AAB) are commonly found in Brazilian industrial ethanolic fermentations, with the genders Lactobacillus and Acetobacter among the most commons. Species such as L. plantarum, L. paraplantarum, L. fermentum, A. tropicalis and A. senegalensis are among the most studied in these groups. This project aimed to perform the genome sequencing and assembly of three bacterial isolates founded in sugarcane juice fermentations plants located in the Piracicaba region, Interior of São Paulo State, Brazil. This study will provide more information about the genetic composition of these microorganisms and will allow studies of their phylogenetic and evolutionary relationships with other bacteria.
Sample: Microbe sample from Lactobacillus plantarum
SAMN13031559 • SRS5526136 • All experiments • All runs
Library:
Name: L10
Instrument: Illumina HiSeq 2500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 3.4M spots, 689.9M bases, 518.4Mb
Run# of Spots# of BasesSizePublished
SRR102919203,415,112689.9M518.4Mb2019-10-16

ID:
9207997

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