show Abstracthide AbstractBrazilian ethanol production industries are not contamination-free environments, and it is common to observe other microorganisms than Saccharomyces cerevisiae. Probably the main origin of these microorganisms is the sugarcane juice itself, since the industrial environment is highly selective and marked by stresses such as high temperatures and sugar concentrations. Theoretically, only organisms adapted to these conditions, with a long history of coevolution with S. cerevisiae yeasts, would be able to tolerate these environments. Lactic Acid Bacteria (LAB) and Acetic Acid Bacteria (AAB) are commonly found in Brazilian industrial ethanolic fermentations, with the genders Lactobacillus and Acetobacter among the most commons. Species such as L. plantarum, L. paraplantarum, L. fermentum, A. tropicalis and A. senegalensis are among the most studied in these groups. This project aimed to perform the genome sequencing and assembly of three bacterial isolates founded in sugarcane juice fermentations plants located in the Piracicaba region, Interior of São Paulo State, Brazil. This study will provide more information about the genetic composition of these microorganisms and will allow studies of their phylogenetic and evolutionary relationships with other bacteria.