WGS of L. plantarum: D. melanogastor gut - SRA

SRX9508708: WGS of L. plantarum: D. melanogastor gut
1 ILLUMINA (Illumina HiSeq 2500) run: 1.8M spots, 751.6M bases, 366.3Mb downloads

Design: The DNA was extracted using the Qiagen DNeasy Powerlyzer Microbial Kit. DNA was then fragmented to 500 bp using NEBNext dsDNA Fragmentase (M0348L) and end-repaired with NEBNext Ultra II End Repair/dA-Tailing module (E7546L). Fragments were ligated to Illumina sequencing adapters with NEBNext Ultra II Ligation Module (E7595L) and size selected to roughly 500 bp. The ligated target sequences were enriched with KAPA Library Amplification kit (KK2621), again size selected to 500 bp, and normalized to 5ng/uL using Qubit measurements. The library was then sequenced on a partial lane of Illumina HiSeq 2500 using 250bp paired-end sequencing.

Submitted by: Brigham Young University

Study: Drosophila Model of Parkinson's

PRJNA678409 • SRP292562 • All experiments • All runs

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Establishing a genomic resource for understanding microbial contributions to Parkinson's Disease.

Sample:

SAMN16795837 • SRS7717240 • All experiments • All runs

Organism: Lactiplantibacillus plantarum

Library:

Name: g22

Instrument: Illumina HiSeq 2500

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 1.8M spots, 751.6M bases, 366.3Mb

Run	# of Spots	# of Bases	Size	Published
SRR13060588	1,780,340	751.6M	366.3Mb	2020-11-14

ID:: 12424973

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