WGS of Campylobacter jejuni strain GB60 - SRA

SRX8387872: WGS of Campylobacter jejuni strain GB60
1 ILLUMINA (Illumina MiSeq) run: 499,844 spots, 244.1M bases, 144.7Mb downloads

Design: Illumina MiSeq Illumina libraries were prepared for all strains using the KAPA Low-Throughput Library Preparation Kit with Standard PCR Amplification Module (Kapa Biosystems, Wilmington, MA), following manufacturer's instructions except for the following changes: 750 ng DNA was sheared at 30 psi for 40 s and size selected to 700-770 bp following Illumina protocols. Standard desalting TruSeq LT and PCR Primers were ordered from Integrated DNA Technologies (Coralville, IA) and used at 0.375 and 0.5 M final concentrations, respectively. PCR was reduced to 3-5 cycles. Libraries were quantified using the KAPA Library Quantification Kit (Kapa), except with 10 l volume and 90-s annealing/extension PCR, then pooled and normalized to 4 nM. Pooled libraries were requantified by ddPCR on a QX200 system (Bio-Rad), using the Illumina TruSeq ddPCR Library Quantification Kit and following manufacturer's protocols, except with an extended 2-min annealing/extension time. The libraries were sequenced 2 250 bp paired end v2 on a MiSeq instrument (Illumina) at 13.5 pM, following manufacturer's protocols.

Submitted by: United States Department of Agriculture, Agricultural Research Service

Study: Campylobacter jejuni subsp. jejuni Raw sequence reads

PRJNA634604 • SRP263184 • All experiments • All runs

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Campylobacter jejuni raw sequence reads

Sample: WGS of Campylobacter jejuni strain GB60

SAMN15004776 • SRS6701405 • All experiments • All runs

Organism: Campylobacter jejuni

Library:

Name: CjGB60

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 499,844 spots, 244.1M bases, 144.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR11837462	499,844	244.1M	144.7Mb	2020-06-30

ID:: 10906873

SRA

Sequence Read Archive

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