SRX14503544: Pseudomonas aeruginosa strain FB_L0665
1 BGISEQ (BGISEQ-500) run: 19.5M spots, 1.9G bases, 1.1Gb downloads
Design: Bacterial genomic DNA was extracted using the GeneJET Genomic DNA Purification kit (Thermo Fisher). For samples sequenced at BGI, the bacterial genome was fragmented by Covaris 55 L series Ultrasonicator, and used to construct paired-end libraries with an insert size of 200-400 bp. Bacterial genomes were sequenced on the BGISEQ-500 (MGI, BGI-Senzhen) platform, generating 1.4-2.0 Gb sequencing data for each sample with sequencing depth >100x. Reads were checked for contamination using kraken2 and only considered for further analysis if >90% of the reads identified as P. aeruginosa. Quality control of the raw data was performed using FastQC with default parameters. For samples sequenced at MiGS, XXX. Reads obtained for all samples were assembled using Unicycler and the assembly quality was assessed using assembly-stats.v1.0.1 and BUSCO.v4 (pseudomonodales_odb10).
Submitted by: TU Delft
Study:
Pseudomonas aeruginosa anti-phage defense system investigation bacterial strainsshow Abstracthide AbstractInvestigation of the effects of defense systems on phage infectability in Pseudomonas aeruginosa
Library:
Name: L0665
Instrument: BGISEQ-500
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
1 run, 19.5M spots, 1.9G bases, 1.1Gb