WGS of Enterobacter cloacae: asymmetrical read lengths: Sample OLC-16... - SRA

SRX862832: WGS of Enterobacter cloacae: asymmetrical read lengths: Sample OLC-1682
1 ILLUMINA (Illumina MiSeq) run: 1.4M spots, 397M bases, 248.7Mb downloads

Design: "Isolates were cultured on nutrient agar (Difco, Becton, Dickinson & Co) overnight (14-16 hrs) at 37 degree C, and genomic DNA was extracted from single colonies using the Maxwell 16 Cell LEV DNA Purification kit (Promega, Madison, WI). DNA was quantified using the Quant-iT High-Sensitivity DNA Assay Kit (Life Technologies Inc., Burlington, ON). Sequencing libraries were constructed from 1 ng of gDNA using the Nextera XT DNA sample preparation kit (Illumina, Inc., San Diego, CA) and the Nextera XT index kit (Illumina, Inc.)."

Submitted by: Canadian Food Inspection Agency

Study: GeneSippr

PRJNA273275 • SRP052916 • All experiments • All runs

show Abstracthide Abstract

Genome assemblies of Escherichia coli and Enterobacter cloacae strains processed with the GeneSippr pipeline

Sample:

SAMN03292329 • SRS833789 • All experiments • All runs

Organism: Enterobacter ludwigii

Library:

Name: 2014-SEQ-327

Instrument: Illumina MiSeq

Strategy: WGS

Source: GENOMIC

Selection: RANDOM

Layout: PAIRED

Spot descriptor:

1 forward22 reverse

Experiment attributes:

alignment_software: N/A

Runs: 1 run, 1.4M spots, 397M bases, 248.7Mb

Run	# of Spots	# of Bases	Size	Published
SRR1783846	1,446,902	397M	248.7Mb	2015-04-28

ID:: 1235872

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