Draft genome Lactobacillus rhamnosus OSU-PECh-69 - SRA

SRX8944637: Draft genome Lactobacillus rhamnosus OSU-PECh-69
1 ILLUMINA (Illumina NovaSeq 6000) run: 40.3M spots, 12.2G bases, 3.6Gb downloads

Design: Library construction was prepared by random fragmentation of the gDNA followed by 5and 3adapter ligation (TruSeq DNA PCR-Free 350; Illumina, USA). In additional, tagmentation was performed in a single step to increase the efficiency of the library preparation process. To verify the size of adapter-ligated fragments a PCR was performed (TapeStation 2200; Agilent, USA). For cluster generation, the library was loaded into a flow cell where fragments are captured on a lawn of surface-bound oligos complementary to the library adapters. Each fragment was amplified in clonal clusters through bridge amplification. Illumina SBS technology NovaSeq6000 S4 system (Illumina, USA) was used to perform the sequencing

Submitted by: The Ohio State University

Study: Lactobacillus rhamnosus strain:OSU-PECh-69 Genome sequencing and assembly

PRJNA634258 • SRP266222 • All experiments • All runs

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The strain was isolated from cheese and show strong antibacterial activity against Gram-positive and Gram-negative microorganisms. The aim is to find the gene cluster responsable to produce the antibacterial activity

Sample: Model organism or animal sample from Lactobacillus rhamnosus OSU-PECh-69

SAMN14987738 • SRS6784787 • All experiments • All runs

Organism: Lacticaseibacillus rhamnosus

Library:

Name: OSU-PECh-69

Instrument: Illumina NovaSeq 6000

Strategy: WGS

Source: GENOMIC

Selection: PCR

Layout: SINGLE

Runs: 1 run, 40.3M spots, 12.2G bases, 3.6Gb

Run	# of Spots	# of Bases	Size	Published
SRR12450178	40,271,142	12.2G	3.6Gb	2020-08-13

ID:: 11627730

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