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BioSample

National Center for Biotechnology Information

Organism: Lactobacillus delbrueckii
cellular organisms; Bacteria; Terrabacteria group; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae; Lactobacillus

Attributes

isolate	UW_AWMP_LAC1_4
collection date	2022-11-10
broad-scale environmental context	bioreactor [ENVO:00002123]
local-scale environmental context	anaerobic bioreactor [ENVO:00002124]
environmental medium	biological waste material [ENVO:01000373]
geographic location	USA: Madison, WI
isolation source	cottage cheese acid whey and ultra-filtered milk permeate fermenting bioreactor operated as a continuously stirred tank reactor with a six day solids and hydraulic retention time, under anaerobic conditions, at a pH of 5.5, at 50 C, and was fed cottage cheese acid whey and ultra-filtered milk permeate in a 50/50 v/v mixture. This sample was from the 140th day of operation.
latitude and longitude	43.07381396 N 89.41403084 W
altitude	268 m
collection method	DNeasy PowerSoil Pro kit (QIAGEN, Hilden, Germany)
dissolved oxygen	anaerobic conditions
experimental factor	Temperature = 50 C
pH	5.5
reference for biomaterial	3300062705_20
sample collection device or method	Syringe
sample material processing	Biomass centrifuged 10 min 10,000xg, aliquot frozen for DNA extraction
sample size	2 mL
sample storage location	Freezer
sample storage temperature	-80 C
sample volume or weight for DNA extraction	2 mL
source material identifiers	B3_r2
temperature	50 C
sample type	metagenomic assembly
metagenome source	bioreactor metagenome
derived from	SAMN38199228
metagenomic	1
environmental-sample	1
BioProject (physical sample)	PRJNA1038729
Project Accession (physical sample)	SRP473597
Run Accession (physical sample)	SRR26910050