GEO DataSets

(Submitter supplied) Methanogens inhabit euxinic (sulfide-rich) or ferruginous (iron-rich) environments that promote the precipitation of transition metals as metal sulfides, such as pyrite, reducing metal or sulfur availability. Such environments have been common throughout Earth’s history raising the question as to how anaerobes obtain(ed) these elements for the synthesis of enzyme cofactors. Here, we show a methanogen can synthesize molybdenum nitrogenase metallocofactors from pyrite as the source of iron and sulfur, enabling nitrogen fixation. more...

Organism:

Methanococcus maripaludis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32793

12 Samples

Download data: TXT

(Submitter supplied) Using the high-throughput strand-specific RNA-seq, we performed differential transcriptome analysis between strains S2 and ▽aCPSF1 Using the Term-seq method, we meatured the RNA 3'-end of strains S2 and ▽aCPSF1

Organism:

Methanococcus maripaludis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27854

10 Samples

Download data: TXT

(Submitter supplied) Methanococcus maripaludis is a methanogenic Archaea that conserves energy from molecular hydrogen to reduce carbon dioxide to methane. Chemostat grown cultures limited for phosphate or leucine were compared to determine the regulatory response to leucine limitation. Keywords: archaea, hydrogen, leucine, phosphate, nutrient limitation, growth rate, methanogen

Organism:

Methanococcus maripaludis

Type:

Expression profiling by array

Platform:

GPL2509

16 Samples

Download data: SPT

(Submitter supplied) Methanococcus maripaludis is a methanogenic Archaea that conserves energy from molecular hydrogen to reduce carbon dioxide to methane. Chemostat grown cultures limited for hydrogen, phosphate, or leucine were compared to determine the regulatory response to hydrogen limitation. This was done by comparing hydrogen limited cultures to both leucine limited and phosphate limited cultures. Slow and rapid growing samples limited for either hydrogen or phosphate were compared to determine the regulatory effects of growth rate. more...

Organism:

Methanococcus maripaludis

Type:

Expression profiling by array

Platform:

GPL2509

68 Samples

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(Submitter supplied) Methanococcus maripaludis is a methanogenic archaeon. Within its genome, there are two operons for membrane associated hydrogenases, eha and ehb. To investigate the regulation of ehb on the cell, an S40 mutant was constructed in such a way that a portion of the ehb operon was replaced by pac cassette in the wild type parental strain S2 (done by Whitman's group at the University of Georgia). Four samples of each strain were grown in batch culture. more...

Organism:

Methanococcus maripaludis

Type:

Expression profiling by array

Platform:

GPL2509

16 Samples

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(Submitter supplied) Methanococcus maripaludis is a methanogenic archaeon. Within its genome, there are two operons for membrane associated hydrogenases, eha and ehb. To investigate the regulation of ehb on the cell, an S40 mutant was constructed in such a way that a portion of the ehb operon was replaced by pac cassette in the wild type parental strain S2 (done by Whitman's group at the University of Georgia). The S40 and S2 strains were grown in 14N and 15N media with acetate separately. more...

Organism:

Methanococcus maripaludis; Methanococcus maripaludis S2

Type:

Other

Platforms:

GPL2103 GPL2102

2 Samples

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(Submitter supplied) Methanococcus maripaludis array based on partial genome sequence. Covers 1597 of 1722 predicted orfs. PCR products spotted in duplicate on glass slides. Average length 500 bp. Keywords: archaea, methanogen, anaerobic Platform accession ID: NC_005791 Protocol: PCR products spotted in duplicate on glass slides.

Organism:

Methanococcus maripaludis

3 Series

100 Samples

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(Submitter supplied) Methanococcus maripaludis qualitative proteomics using ion trap mass spectrometry. Covers 1289 of 1722 predicted ORFs.

Organism:

Methanococcus maripaludis

1 Series

1 Sample

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