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| Status |
Public on Nov 06, 2018 |
| Title |
Global transcriptomic response of modified Bacillus anthracis over expressing protective antigen |
| Organism |
Bacillus anthracis str. Ames |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study we focused on understanding the effect of over expression of recombinant protective antigen (rPA) on the Bacillus anthracis metabolism and genes expression with the purpose of improving the production of this recombinant toxin in particular and recombinant proteins in general. To achieve this goal controlled growth of the B. anthracis ames strain transformed with pYS5 (PA producer) plasmid encoding protective antigen and the corresponding empty vector pSW4 (non-producer) were performed and protein production, bacterial growth characteristics and gene transcription pattern were analyzed. Controlled condition batch runs were performed for both strains and samples from early log (OD 3), log (OD 10) and late log (OD 16) phase were used for gene expression profiling. We found that most of the genes belonging to transport, TCA, glycolysis, PPP and amino acid biosynthesis were up-regulated in the lag phase samples which help the cell coping with the increased requirements of nutrient and energy for rPA expression. These pathways are down-regulated in the log and latelog phase as cellular stress response onsets, which is reflected in the decreased specific growth rate.
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| Overall design |
Time point mRNA profiling of Bacillus anthracis ames strain BH500 transformed with pYS5 (PA producer) and the corresponding empty vector pSW4 (non-producer).
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| Contributor(s) |
Sharma AK, Shiloach J, Leppla SH, Pomerantsev A |
| Citation(s) |
30382110 |
| Submission date |
Jan 09, 2018 |
| Last update date |
Nov 06, 2018 |
| Contact name |
Ashish K Sharma |
| E-mail(s) |
ashish.sharma@nih.gov, ashish-sharma@idexx.com, ashish.kumar07@gmail.com
|
| Phone |
3014022981
|
| Organization name |
National Institute of Health
|
| Department |
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
|
| Lab |
Biotechnology Core
|
| Street address |
14 Service Rd West Building 14A Room 176 MS-5522
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL24488 |
Illumina HiSeq 2500 (Bacillus anthracis str. Ames) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA429267 |
| SRA |
SRP128581 |
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