|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Mar 11, 2019 |
Title |
The role of archaeal histones in gene expression - a synthetic biology perspective [Methanothermus fervidus] |
Organism |
Methanothermus fervidus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
Since their discovery, archaea have not only proven a fascinating domain in their own right, but also helped us understand the evolution and function of molecular components they share with bacteria or eukaryotes. Archaeal histones are homologous to their eukaryotic counterparts, but operate in a less constrained bacterial-like cellular environment and their role in transcription and genome function remains obscure. In order to understand how archaeal histones affect transcriptional processes, we induced expression of the two histones from the archaeon Methanothermus fervidus in a naive bacterial system (E. coli) that has not evolved to integrate this kind of proteins. We show, using a series of MNase digestion experiments, that these histones bind the bacterial genome and wrap DNA in vivo in a pattern consistent with a previously proposed multimerisation model, in a similar pattern observed natively. We correlate genome-wide occupancy maps and gene expression profiles in different phases of growth to show that – although expression of archaeal histones triggers morphological changes in E. coli – there appears to only be an indirect effect on transcription. Since their discovery, archaea have not only proven a fascinating domain in their own right, but also helped us understand the evolution and function of molecular components they share with bacteria or eukaryotes. Archaeal histones are homologous to their eukaryotic counterparts, but operate in a less constrained bacterial-like cellular environment and their role in transcription and genome function remains obscure. In order to understand how archaeal histones affect transcriptional processes, we induced expression of the two histones from the archaeon Methanothermus fervidus in a naive bacterial system (E. coli) that has not evolved to integrate this kind of proteins. We show, using a series of MNase digestion experiments, that these histones bind the bacterial genome and wrap DNA in vivo in a pattern consistent with a previously proposed multimerisation model, in a similar pattern observed natively. We correlate genome-wide occupancy maps and gene expression profiles in different phases of growth to show that – although expression of archaeal histones triggers morphological changes in E. coli – there appears to only be an indirect effect on transcription.
|
|
|
Overall design |
Correlation between genome-wide occupancy of the archaeal histones HMfA and HMfB in E.coli and transcriptional changed triggered by their expression, coupled with comparison between the histones' binding patterns in Methanothermus fervidus and E. coli.
|
|
|
Contributor(s) |
Rojec M, Warnecke T |
Citation(s) |
31692448, 31710291 |
Submission date |
Mar 01, 2019 |
Last update date |
Dec 03, 2019 |
Contact name |
Tobias Warnecke |
E-mail(s) |
molecular.systems.laboratory@gmail.com
|
Organization name |
Imperial College
|
Street address |
Du Cane Road
|
City |
London |
ZIP/Postal code |
W12 0NN |
Country |
United Kingdom |
|
|
Platforms (1) |
GPL26241 |
Illumina MiSeq (Methanothermus fervidus) |
|
Samples (6)
|
|
This SubSeries is part of SuperSeries: |
GSE127680 |
The role of archaeal histones in gene expression - a synthetic biology perspective |
|
Relations |
BioProject |
PRJNA525016 |
SRA |
SRP187183 |
Supplementary file |
Size |
Download |
File type/resource |
GSE127678_MetFerv_exp_trimmed_merged_uniqueH.bw |
6.6 Mb |
(ftp)(http) |
BW |
GSE127678_MetFerv_st_trimmed_merged_uniqueH.bw |
6.6 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
|
|
|
|
|