 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jul 04, 2019 |
| Title |
Assigning a role for chemosensory signal transduction in Campylobacter jejuni biofilms using a combined omics approach |
| Organism |
Campylobacter jejuni |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Biofilms serve as a protective mechanism for bacteria to cope with environmental stress. Whilst ordinarily a fastidious organism, it has been long suggested that C. jejuni is able to utilise this mode of growth as a way to transmit infection from the avian host to humans. Herein, we undertook a combinatorial approach to examine differential expression of C. jejuni genes and protein abundance during biofilm formation. RNA sequencing and proteomics via quantitative liquid chromatography – tandem mass spectrometry (LC-MS/MS) revealed biofilm growth induced a substantial rearrangement of the C. jejuni transcriptome and proteome, with ~600 genes differentially expressed in biofilms compared to planktonic cells. Biofilm-induced genes / proteins were those involved in iron metabolism and acquisition, cell division, glycan production and attachment, while those repressed were associated with metabolism, amino acid uptake and utilisation, and large tracts of the chemotaxis pathway.
|
| |
|
| Overall design |
Examination of differentially expressed transcripts from planktonic cells and those in a biofilm in Campylobacter jejuni
|
| |
|
| Contributor(s) |
Tram G, Klare WP, Cain JA, Mourad B, Cordwell SJ, Day CJ, Korolik V |
| Citation(s) |
31896623, 32321947 |
| Submission date |
Jul 03, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Greg Tram |
| E-mail(s) |
g.tram@griffith.edu.au
|
| Organization name |
Griffith University
|
| Department |
Institute for Glycomics
|
| Street address |
Parklands Drive
|
| City |
Southport |
| State/province |
Queensland |
| ZIP/Postal code |
4215 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL26884 |
Illumina NextSeq 500 (Campylobacter jejuni) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA552539 |
| SRA |
SRP212896 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE133783_C.jejuni_processed_data_files.xlsx |
130.3 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...