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| Status |
Public on May 27, 2020 |
| Title |
Transcriptome RNA Sequencing Data Sets of Bacillus anthracis Vollum DhtrA and Parental Isogenic Wild-Type Strains under Oxidative Stress Conditions |
| Organism |
Bacillus anthracis str. Vollum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The High Temperature Requirement (HtrA) chaperone/protease is involved in the stress-response of the anthrax-causing pathogen Bacillus anthracis. Resilience to oxidative stress is essential for manifestation of B. anthracis pathogenicity. We submitt transcriptome data sets detailing global gene expression in B. anthracis wild-type and htrA-disrupted strains following H2O2-induced oxidative stress.
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| Overall design |
B. anthracis parental DVollum (acapsular and nontoxinogenic, referred as wild type, wt) and an htrA disrupted strain, were grown in Brain-Heart Infusion broth at 37°C to mid log phase and split into twin cultures in the presence or absence of 3 mM H2O2. Cells were collected from the initial culture before treatment (WT denoted sample 1 and DhtrA denoted sample 2) and 10 minutes post treatment-without H2O2-(WT sample denoted 3 and DhtrA denoted sample 5), and with H2O2 (WT sample denoted 4 and DhtrA denoted sample 6). The project includes 14 sets of transcriptomic data: Samples 1, 2, 3 and 5, each one in biological douplicates; Samples 4 and 6, in biological triplicates. Total RNA was extracted using the RNeasy kit (Qiagen). RNA-seq was performed in-house (IIBR, Ness-Zionna, Israel).
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| Contributor(s) |
Cohen-Gihon I, Israeli O, Elia U, Zaide G, Israeli M, Beth-Din A, Zvi A, Cohen O, Chitlaru T |
| Citation(s) |
32855245, 33265965 |
| Submission date |
May 26, 2020 |
| Last update date |
Jan 04, 2021 |
| Contact name |
Inbar Cohen Gihon |
| E-mail(s) |
inbarg@iibr.gov.il
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| Organization name |
Israel Institute For Biological Research
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| Department |
Biochemistry
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| Street address |
Reuven 24
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| City |
Ness Ziona |
| ZIP/Postal code |
14100 |
| Country |
Israel |
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| Platforms (1) |
| GPL28578 |
Illumina Genome Analyzer IIx (Bacillus anthracis str. Vollum) |
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| Samples (14)
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| GSM4568574 |
WT before treatment with H2O2 1st duplicate |
| GSM4568575 |
WT before treatment with H2O2 2nd duplicate |
| GSM4568576 |
DhtrA mutant before treatment with H2O2 1st duplicate |
| GSM4568577 |
DhtrA mutant before treatment with H2O2 2nd duplicate |
| GSM4568578 |
WT in the absence of H2O2 1st duplicate |
| GSM4568579 |
WT in the absence of H2O2 2nd duplicate |
| GSM4568580 |
WT 10 minutes after treatment with 3mM H2O2 1st triplicate |
| GSM4568581 |
WT 10 minutes after treatment with 3mM H2O2 2nd triplicate |
| GSM4568582 |
WT 10 minutes after treatment with 3mM H2O2 3rd triplicate |
| GSM4568583 |
DhtrA mutant in the absence of H2O2 1st duplicate |
| GSM4568584 |
DhtrA mutant in the absence of H2O2 2nd duplicate |
| GSM4568585 |
DhtrA 10 minutes after treatment with 3mM H2O2 1st triplicate |
| GSM4568586 |
DhtrA 10 minutes after treatment with 3mM H2O2 2nd triplicate |
| GSM4568587 |
DhtrA 10 minutes after treatment with 3mM H2O2 3rd triplicate |
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| Relations |
| BioProject |
PRJNA635127 |
| SRA |
SRP264814 |
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