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| Status |
Public on Jun 25, 2009 |
| Title |
Halobacterium salinarum NRC-1 total RNA hybridization of TFBd overexpression versus Reference sample |
| Organism |
Halobacterium salinarum NRC-1 |
| Experiment type |
Expression profiling by genome tiling array
|
| Summary |
Total RNA hybridizations of Halobacterium salinarum NRC-1 total RNA versus a strain overexpressing TFBd were compared to verify the transcriptome landscape changes. Comparison with TFBd binding sites localized using ChIP-chip data and MeDiChI algorithm (Reiss et al, 2008) allowed observation that TF-binding in the middle of coding sequences can also result in transcription initiation or termination internal to a single annotated protein-coding gene.
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| Overall design |
Total RNA sample of plasmid based expression of Cmyc tagged transcription factor TFBd grown in standard growth media with mevinolin.
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| Contributor(s) |
Facciotti MT, Pan M, Koide T, Reiss DJ, Baliga NS |
| Citation(s) |
19536208, 25806405 |
| Submission date |
Apr 23, 2009 |
| Last update date |
Jun 07, 2019 |
| Contact name |
Tie Koide |
| E-mail(s) |
tiekoide@gmail.com
|
| Organization name |
Institute for Systems Biology
|
| Street address |
1441 N 34th street
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL8468 |
NimbleGen Halobacterium 385K tiling array |
|
| Samples (2) |
| GSM396427 |
Total RNA sample from Cmyc tagged TFBd, Halobacterium NRC-1. Array 77972 |
| GSM396429 |
Total RNA sample from Cmyc tagged TFBd, Halobacterium NRC-1. Array 95767 |
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| This SubSeries is part of SuperSeries: |
| GSE13150 |
Prevalence of transcription promoters within archaeal operons and coding sequences |
|
| Relations |
| BioProject |
PRJNA116867 |
