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| Status |
Public on Aug 25, 2021 |
| Title |
Transcriptional profile of Halobacterium salinarum histone [RNA-seq] |
| Organism |
Halobacterium salinarum |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Unlike the characterised histone proteins of a number of methanogenic and themophilic archaea, previous research indicated that HpyA, the sole histone encoded in the model halophile Halobacterium salinarum, is not involved in DNA packaging. Instead, it was found to have widespread but subtle effects on gene expression, and affect cell morphology. Here we report that HpyA is important for growth in reduced salinity and regulates gene expression of ion uptake and nucleotide metabolism pathways in a salt-dependent manner. Analysis of the genome-wide binding pattern of HpyA reveals that its binding is dependent on external salt concentration as well as growth phase. Combining the transcriptomic and ChIP-Seq data suggests a role for HpyA as a regulator of the expression of ion uptake genes as well as a co-regulator of other genes of diverse functions in a salt-dependent manner. In this GEO series , we use RNA-Seq to study the transcriptonal effect of HpyA in optimal (4.2M NaCl) and reduced (3.4M) during exponential growth phase.
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| Overall design |
Examination of HpyA transcriptionall profile in the halophilic archaeon Halobacterium salinarum in mid-logarithmic phase of growth, in optimal (4.2M NaCl) and reduced (3.4M NaCl) salt concentrations, comparing Δura3 ("WT") and ΔhpyA ("KO") strains
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| Contributor(s) |
Schmid A, Sakrikar S |
| Citation(s) |
34883507 |
| BioProject |
PRJNA703048 |
| Submission date |
Aug 20, 2021 |
| Last update date |
Jan 07, 2022 |
| Contact name |
Amy Schmid |
| E-mail(s) |
amy.schmid@duke.edu
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| Organization name |
Duke University
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| Street address |
3242 French Family Science Center
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| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL30532 |
Illumina Novaseq 6000 (Halobacterium salinarum) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE182514 |
Binding and Transcriptional profile of Halobacterium salinarum histone |
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