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| Status |
Public on Jan 01, 2011 |
| Title |
Bile stress response in probiotic Lactobacillus rhamnosus GG |
| Platform organism |
Lacticaseibacillus rhamnosus GG |
| Sample organism |
Lacticaseibacillus rhamnosus |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of probiotic Lactobacillus rhamnosus strain GG mid-exponential pH-controlled bioreactor cultures before and after exposure to bovine bile (0.2% ox gall). Keywords: bile, stress response
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| Overall design |
Cell samples from four biological replicates were harvested right before (time point 0 min) and 10, 30 and 120 min after bile treatment. Each sample was compared to a common reference sample (time point 0 min, mid-exponential growth phase Lactobacillus rhamnosus GG cultures). A total of 12 hybridizations were performed using balanced dye-swap design. Dyes were balanced between compared sample pairs and between biological replicates.
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| Contributor(s) |
Koskenniemi K, Laakso K, Koponen J, Kankainen M, Greco D, Auvinen P, Savijoki K, Nyman TA, Surakka A, Salusjärvi T, de Vos WM, Tynkkynen S, Kalkkinen N, Varmanen P |
| Citation(s) |
21078892 |
| Submission date |
Jun 23, 2010 |
| Last update date |
Mar 22, 2012 |
| Contact name |
Kati Laakso |
| E-mail(s) |
kati.laakso@valio.fi
|
| Organization name |
Valio Ltd
|
| Department |
R&D
|
| Street address |
Meijeritie 4
|
| City |
Helsinki |
| ZIP/Postal code |
00370 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL10580 |
Lactobacillus rhamnosus GG whole genome microarray 4X44K |
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| Samples (12)
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| GSM559525 |
10 minutes bile exposure replicate A |
| GSM559526 |
10 minutes bile exposure replicate B |
| GSM559527 |
10 minutes bile exposure replicate C |
| GSM559528 |
10 minutes bile exposure replicate D |
| GSM559529 |
30 minutes bile exposure replicate A |
| GSM559530 |
30 minutes bile exposure replicate B |
| GSM559531 |
30 minutes bile exposure replicate C |
| GSM559532 |
30 minutes bile exposure replicate D |
| GSM559533 |
120 minutes bile exposure replicate A |
| GSM559534 |
120 minutes bile exposure replicate B |
| GSM559535 |
120 minutes bile exposure replicate C |
| GSM559536 |
120 minutes bile exposure replicate D |
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| Relations |
| BioProject |
PRJNA128629 |
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