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| Status |
Public on Mar 11, 2024 |
| Title |
Analysis of ΔcrcZ suppressor mutations |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The ΔcrcZ mutant shows constitutive repression of all crc/Hfq target genes in the translation. Therefore, it presents a hyper‐repressed phenotype (more repressed than the parental strain) in the acquisition of secondary carbon sources, even though the preferred carbon source is not present.
Δcrc is more sensitive to antibiotics, more susceptible to oxidative stress, and less motile than the parental strain.
The ΔcrcZ strain is more motile and more resistant to antibiotics than the parental strain. However, it presents a susceptibility profile to oxidative stress that is very similar to that of the parental strain.
When the ΔcrcZ mutant is seeded on minimal medium with a secondary carbon source it grows poorly. However, the appearance of colonies that grow similarly to the parental strain is observed. These colonies are formed from mutants derived_expression.tsv from the ΔcrcZ strain that are "revertants" of this repression phenotype in the assimilation of the secondary carbon source, and generally "pseudorevertants" of the ΔcrcZ mutation, or present a "mutation "suppressor" of the crcZ deletion. 4 mutant clones resistant to FAA (FAA resistant, present catabolic repression) and sensitive clones (FAA sensible, do not present catabolic repression) were analyzed.
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| Overall design |
We performed gene expression profiling analysis using data obtained from RNA-seq of 11 different Pseudomonas aerginosa strains
Comparative gene expression profiling analysis of RNA-seq data for Pseudomonas aeruginosa PAO1, its Δcrc ΔcrcZ mutants and pseudorevertent strains from ΔcrcZ
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| Contributor(s) |
Corona F, Martinez JL |
| Citation missing |
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| BioProject |
PRJNA934266 |
| Submission date |
Feb 16, 2023 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Rafael Torres |
| E-mail(s) |
rafael.torres@cnb.csic.es
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| Organization name |
CNB
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| Department |
Bioinformatics for Genomics and Proteomics (BioinfoGP)
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| Lab |
Bioinformatics for Genomics and Proteomics (BioinfoGP)
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| Street address |
Darwin 3
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| City |
Madrid |
| State/province |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL15987 |
Illumina Genome Analyzer IIx (Pseudomonas aeruginosa PAO1) |
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| Samples (11)
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