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| Status |
Public on Feb 18, 2024 |
| Title |
Dynamic modification of tRNAs determines pathogenicity in the opportunistic pathogen Pseudomonas aeruginosa [RNA-Seq and Ribo-Seq] |
| Organism |
Pseudomonas aeruginosa UCBPP-PA14 |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The overall success of bacterial pathogens depends on the coordinated expression of virulence determinants. Regulatory circuits that drive pathogenesis are complex, multilayered and incompletely understood. Here, we reveal that alterations in tRNA modifications define pathogenic phenotypes in the opportunistic pathogen Pseudomonas aeruginosa. We demonstrate that the enzymatic activity of GidA leads to the introduction of a carboxymethylaminomethyl modification in selected tRNAs. Modifications at the wobble uridine base (cmnm5U34) of the anticodon alter ribosome occupancy of transcripts enriched in cognate codons of such tRNAs. Specifically, in P. aeruginosa the presence of GidA-dependent tRNA modifications influenced expression of genes encoding virulence regulators, leading to a cellular proteomic shift towards pathogenic and well-adapted physiological states. Our approach of profiling the consequences of chemical tRNA modifications is general in concept. It provides a paradigm of how tRNA modifications govern gene expression programs and regulate phenotypic outcomes responsible for bacterial adaption to changing and challenging habitats prevailing in the host niche.
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| Overall design |
Pseudomonas aeruginosa UCBPP-PA14 mutant strains were planktonically cultured in LB to an OD600 of 0.4 (ribo seq experiment) or 2 (RNA seq experiment).domonas aeruginosa UCBPP-PA14 wild type and mutants strains cultivated in LB
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| Contributor(s) |
Krueger J, Preusse M, Trinler NO, Frommeyer Y, Doberenz S, Lorenz A, Grobe S, Müsken M, Svensson SL, Imsiecke A, Weiss S, Kaever V, Pich A, Sharma CM, Ignatova Z, Haeussler S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 24, 2023 |
| Last update date |
Feb 19, 2024 |
| Contact name |
Susanne Häußler |
| E-mail(s) |
Susanne.Haeussler@helmholtz-hzi.de
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| Organization name |
Twincore
|
| Department |
Molecular Bacteriology
|
| Street address |
Feodor-Lynen-Str. 7
|
| City |
Hanover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL27892 |
Illumina NovaSeq 6000 (Pseudomonas aeruginosa UCBPP-PA14) |
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| Samples (12)
|
| GSM7421806 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 1 |
| GSM7421807 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 2 |
| GSM7421808 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 3 |
| GSM7421809 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 1 |
| GSM7421810 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 2 |
| GSM7421811 |
RNASeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 3 |
| GSM7421812 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 1 |
| GSM7421813 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 2 |
| GSM7421814 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tngidA 3 |
| GSM7421815 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 1 |
| GSM7421816 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 2 |
| GSM7421817 |
RiboSeq of Pseudomonas aeruginosa UCBPP-PA14 tnladS 3 |
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| This SubSeries is part of SuperSeries: |
| GSE149306 |
Dynamic modification of tRNAs determines pathogenicity in the opportunistic pathogen Pseudomonas aeruginosa |
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| Relations |
| BioProject |
PRJNA975827 |
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