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| Status |
Public on Oct 10, 2012 |
| Title |
Comparison of the response to bile of Lactobacillus casei BL23 and its derivative strain TC01 |
| Platform organism |
Lacticaseibacillus paracasei ATCC 334 |
| Sample organism |
Lacticaseibacillus casei |
| Experiment type |
Expression profiling by array
|
| Summary |
The response to bile of Lactobacillus casei BL23 and its derivative strain TC01 was investigated. TC01 strain carries a complete deletion of gene LCABL_02080 which encodes a two component signal transduction response regulator.
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| Overall design |
Lactobacillus casei BL23 and TC01 strains were grown in MRS medium at 37C without shaking until O.D. 595 nm. reached 0.5. The cultures were then two fold diluted with prewarmed MRS medium (control) or MRS supplemented with 0.2% bovine bile (treatment) and incubation continued for 45 min. Cells were then harvested and total RNA purified.
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| Contributor(s) |
Zúñiga M, Alcántara C |
| Citation(s) |
22322960 |
| Submission date |
Nov 17, 2010 |
| Last update date |
Jan 09, 2013 |
| Contact name |
Manuel Zúñiga |
| E-mail(s) |
btcman@iata.csic.es
|
| Phone |
34 963900022
|
| Fax |
34 963636301
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| Organization name |
Instituto de Agroquímica y Tecnología de Alimentos
|
| Department |
Biotecnología
|
| Lab |
Bacterias lácticas y Probióticos
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| Street address |
Avda. Profesor Agustín Escardino 7
|
| City |
Paterna |
| State/province |
Valencia |
| ZIP/Postal code |
46980 |
| Country |
Spain |
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| Platforms (1) |
| GPL11074 |
IATA Lactobacillus casei ATCC 334 2.7k array_v1 |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA133809 |
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