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| Status |
Public on Aug 22, 2011 |
| Title |
Hybridization of Burkholderia multivorans D2095 and D2214 genomic DNA |
| Platform organisms |
Burkholderia cenocepacia J2315; Burkholderia multivorans ATCC 17616 |
| Sample organism |
Burkholderia multivorans |
| Experiment type |
Other
|
| Summary |
Burkholderia cepacia complex (Bcc) comprises opportunistic bacteria infecting hosts such as cystic fibrosis (CF) patients. Bcc long-term infection of CF patient airways has been associated with emergence of phenotypic variation. Here we studied two Burkholderia multivorans clonal isolates (D2095 and D2214) displaying different morphotypes from a chronically infected CF patient in order to evaluate traits development during lung infection. Since the custom array described in platform GPL13356 was based on Burkholderia multivorans ATCC 17616 genome, here we performed a DNA-DNA hybridization to determine which probes of the array hybridize with our test genomes
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| Overall design |
Total DNA was extracted from overnight cultures of the mucoid D2095 and the nonmucoid D2214 isolates grown in LB medium.
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| Contributor(s) |
Silva IN, Ferreira AS, Becker JD, Moreira LM |
| Citation(s) |
21835880 |
| Submission date |
Jul 05, 2011 |
| Last update date |
May 23, 2012 |
| Contact name |
Leonilde Morais Moreira |
| E-mail(s) |
lmoreira@ist.utl.pt
|
| Phone |
+351 218419031
|
| Organization name |
Instituto Superior Tecnico
|
| Department |
Bioengineering
|
| Lab |
Biological Sciences
|
| Street address |
A. Rovisco Pais
|
| City |
Lisboa |
| ZIP/Postal code |
1049-001 |
| Country |
Portugal |
| |
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| Platforms (1) |
| GPL13356 |
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE30568 |
Burkholderia cepacia complex and cystic fibrosis (CF) patients |
|
| Relations |
| BioProject |
PRJNA154825 |
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