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| Status |
Public on Dec 21, 2011 |
| Title |
The Enterococcus faecalis V583 transcriptional profile during survival within macrophages |
| Platform organisms |
Bacillus anthracis; Salmonella enterica subsp. enterica serovar Typhimurium str. LT2; Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Enterococcus faecalis V583; Bacteroides thetaiotaomicron VPI-5482; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organism |
Enterococcus faecalis V583 |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of E. faecalis V583 during intracellular survival compared to V583 grown to mid-log phase (OD 600 = 0.05) in THB + 1% glucose.
In this study we report the complete intracellular E. faecalis V583 transcriptome following infection of RAW264.7 macrophages. During intracellular survival, approximately 45% of the V583 genome was differentially regulated including numerous genes involved in the oxidative stress, heat shock and SOS responses. We observed that the E. faecalis-containing phagosome was limited for glycolytic substrates, nucleotides, amino acids and numerous ions necessary for growth and protein function. Approximately 35% of the genes differentially regulated during survival within macrophages were of hypothetical/unknown function, suggesting that the V583 response to phagocytosis involves many previously unstudied loci. Here, we provide the first comprehensive study elucidating the transcriptional response of E. faecalis to phagocytosis, which may provide new targets for future studies.
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| Overall design |
Bacterial RNA was obtained from RAW264.7 macrophage infected with E. faecalis V583 4, 8 and 12 h post-infection and compared to E. faecalis V583 RNA extracted from mid log growth (OD 600 = 0.05). Control cultures were grown in Todd-Hewett Broth supplemented with 1% glucose.
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| Contributor(s) |
Maddox S, Conway T, Grissom J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 20, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Scott M Maddox |
| E-mail(s) |
scottmaddox@ou.edu
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| Organization name |
University of Oklahoma
|
| Department |
Botany and Microbiology
|
| Lab |
Tyrrell Conway
|
| Street address |
101 David L. Boren Blvd.
|
| City |
Norman |
| State/province |
OK |
| ZIP/Postal code |
73115 |
| Country |
USA |
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| Platforms (1) |
| GPL6702 |
Affymetrix Custom Array - enterocha520147F |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA150497 |
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