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| Status |
Public on Nov 01, 2012 |
| Title |
The AHL- and BDSF-dependent quorum sensing systems control specific and overlapping sets of genes in Burkholderia cenocepacia H111 |
| Organism |
Burkholderia cenocepacia H111 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Quorum sensing in Burkholderia cenocepacia H111 involves two signalling systems that depend on different signal molecules, namely N-acyl homoserine lactones (AHLs) and the diffusible signal factor cis-2-dodecenoic acid (BDSF). Previous studies have shown that AHLs and BDSF control similar phenotypic traits, including biofilm formation, proteolytic activity and pathogenicity. In this study we mapped the BDSF stimulon by RNA-Seq and shotgun proteomics analysis. We demonstrate that a set of the identified BDSF-regulated genes or proteins are also controlled by AHLs, suggesting that the two regulons partially overlap. The detailed analysis of two mutually regulated operons, one encoding three lectins and the other one encoding the large surface protein BapA and its type I secretion machinery, revealed that both AHLs and BDSF are required for full expression, suggesting that the two signalling systems operate in parallel. In accordance with this, we show that both AHLs and BDSF are required for biofilm formation and protease production.
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| Overall design |
Burkholderia cenocepacia BDSF stimulon was determined in complex media using Illumina RNA-Seq
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| Contributor(s) |
Schmid N, Pessi G, Aguilar C, Deng Y, Carlier AL, Grunau A, Omasits U, Zhang L, Ahrens CH, Eberl L |
| Citation(s) |
23185499 |
| Submission date |
Oct 01, 2012 |
| Last update date |
May 15, 2019 |
| Contact name |
Gabriella Pessi |
| E-mail(s) |
gabriella.pessi@uzh.ch
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| Organization name |
University of Zürich
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| Street address |
Winterthurerstrasse 190
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| City |
Zürich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL16128 |
Illumina HiSeq 2000 (Burkholderia cenocepacia H111) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA176443 |
| SRA |
SRP015965 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE41244_RAW.tar |
400.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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