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| Status |
Public on Jan 01, 2014 |
| Title |
Expression data from Burkholderia cenocepacia J2315 hfq and hfq2 mutants |
| Platform organisms |
Burkholderia cenocepacia J2315; Burkholderia multivorans ATCC 17616 |
| Sample organism |
Burkholderia cenocepacia |
| Experiment type |
Expression profiling by array
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| Summary |
Hfq proteins are RNA chaperones that play a critical role in post-transcription regulation of gene expression. Bacteria of the Burkholderia cepacia complex harbor two distinct and functional Hfq proteins, the Hfq and Hfq2. We have previously performed the functional analysis of Hfq and Hfq2 in the pathogen Burkholderia cenocepacia J2315. In order to examine the impacts of each RNA chaperone on the global transcriptome of B. cenocepacia J2315, we performed comparative transcriptome profile of mutants on the hfq and hfq2 genes, using as reference the wild-type strain.
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| Overall design |
For expression profiling, over-night cultures of the Burkholderia cenocepacia J2315 wild-type strain and the isogenic mutants hfq::Tp and Δhfq2 grown in LB medium were diluted to an initial OD640 nm of 0.25 into LB medium. Triplicate samples were cultured at 37ºC with 250 r.p.m. agitation for 16 h and RNA extracted from the three bacterial isolates.
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| Contributor(s) |
Ramos CG, Leitão JH, Becker JD, Moreira LM |
| Citation missing |
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| Submission date |
May 23, 2013 |
| Last update date |
Jan 02, 2014 |
| Contact name |
Leonilde Morais Moreira |
| E-mail(s) |
lmoreira@ist.utl.pt
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| Phone |
+351 218419031
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| Organization name |
Instituto Superior Tecnico
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| Department |
Bioengineering
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| Lab |
Biological Sciences
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| Street address |
A. Rovisco Pais
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| City |
Lisboa |
| ZIP/Postal code |
1049-001 |
| Country |
Portugal |
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| Platforms (1) |
| GPL13356 |
Affymetrix IST_IBB Burkholderia 15K v1.0 (GeneChip BCC1sa520656F) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA205326 |
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