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Series GSE5642

Query DataSets for GSE5642

Status

Public on Aug 29, 2006

Title

Expression Profiling of Heat Stress Effects on Mice Fed Ergot Alkaloids.

Platform organism

Rattus norvegicus

Sample organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Fescue toxicosis affects wild and domestic animals consuming ergot alkaloids contained in tall fescue forage infected with the endophytic fungus, Neotyphodium coenophialum. , When animals are consuming infected fescue forage during periods of elevated ambient temperatures (summer), a range of phenotypic disorders collectively called summer slump is observed. It is characterized by hyperthermia, with an accompanying decrease in feed intake, growth, milk yield and reproductive fitness. Laboratory mice also exhibit symptoms of fescue toxicosis a thermoneutral temperature, as indicated by reduced growth rate and reproductive fitness. Our goal was to characterize the differences in gene expression in liver of mice exposed to summer-type heat stress (HS) and infected fescue (E+) when compared to mice fed infected fescue at thermoneutral temperature (TN). Mice were fed E+ diet under HS (34 ± 1°C; n = 13; E+HS) or thermoneutral (TN) conditions (24 ± 1°C; n = 14; E+TN) for a period of two weeks between 47 to 60 d of age. Genes differentially expressed between E+HS versus E+TN were identified using DNA microarrays. Forty-one genes were differentially expressed between treatment groups. Expressions of eight genes were measured using quantitative real-time PCR.
Genes coding for phase I detoxification enzymes were up-regulated in E+HS mouse liver. This detoxification pathway is known to produce reactive oxidative species. We observed an up-regulation of genes involved in the protection against reactive oxidative species. Key genes involved in de novo lipogenesis and lipid transport were also up-regulated. Finally, genes involved in DNA damage control and unfolded protein responses were down-regulated.
Keywords: Stress response

Overall design

Differential gene expression in groups of mice exposed to E+HS, E+TN conditions were studied using Reference design of microarrays. We had six mice (3 males, 3 females) in each group used in microarray analysis. Two/three technical replicates were done per mice out of which one replicate was done in dye-swap design. Mice within each treatment group were hydridized against an universal mouse reference RNA and the differential gene expression between the groups were assessed indirectly.

Contributor(s)

Bhusari SS, Zhilin L, Hearne LB, Spiers DE, Lamberson WR, Antoniou E

Citation(s)

17093207

Submission date

Aug 26, 2006

Last update date

Mar 16, 2012

Contact name

Sachin S Bhusari

E-mail(s)

sby7d@mizzou.edu