|
| Status |
Public on Jun 07, 2014 |
| Title |
Microarray analysis of genes regulated by SpxA1 and SpxA2 in Streptococcus suis serotype 2 |
| Platform organism |
Streptococcus suis SC84 |
| Sample organism |
Streptococcus suis |
| Experiment type |
Expression profiling by array
|
| Summary |
To reveal the scope of the Spx regulation and explore the differences in the regulatory roles of SpxA1 and SpxA2, the global gene transcription profiles of the WT, ∆spxA1 and ∆spxA2 strains were determined by DNA microarray analysis performed using an Agilent custom-designed oligonucleotide microarray.
|
| |
|
| Overall design |
S. suis strains were grown in TSB with 10% (vol/vol) newborn bovine serum to mid-exponential phase. Total RNA was isolated using an SV total RNA isolation system (Promega), according to the manufacturer’s recommended protocol. RNA concentrations and integrity were determined using an Agilent 2100 Bioanalyzer.Two biological replicates of total RNA from WT strain and three from each mutant strain were used for array.
|
| |
|
| Contributor(s) |
Zheng C, Bei W |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 13, 2014 |
| Last update date |
Jun 10, 2014 |
| Contact name |
Chengkun Zheng |
| E-mail(s) |
zhengchengkun@yzu.edu.cn
|
| Organization name |
Yangzhou University
|
| Street address |
East wenhui road No.48
|
| City |
Yangzhou |
| ZIP/Postal code |
225009 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18421 |
Agilent-057949 Streptococcus_suis_SC84 8X15K Microarray (Probe Name version) |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA244470 |
Less...
More...