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| Status |
Public on Aug 04, 2014 |
| Title |
RNA-Seq analysis reveals a six-gene SoxR regulon in Streptomyces coelicolor |
| Organism |
Streptomyces coelicolor |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report 6 genes that were found to be regulated by SoxR in stationary phase when the bacteria produce the antibiotic actinorhodin. Five of these genes are previously confirmed SoxR targets; the sixth is a novel SoxR-target.
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| Overall design |
RNA was obtained from 1-day or 3-day old wild type or soxR null mutant strains grown on R2YE agar complex medium. Biological replicates were obtained for 3 day-old samples. RNA was sequenced in an Illumina HiSeq 2000 sequencer for single reads of 100 bp and mapped to the S. coelicolor M145 genome.
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| |
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| Contributor(s) |
Chander M, Naseer N, Shapiro JA |
| Citation(s) |
25162599 |
| Submission date |
May 05, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Joshua A Shapiro |
| E-mail(s) |
joshua.shapiro@brynmawr.edu
|
| Organization name |
Bryn Mawr Collete
|
| Department |
Biology
|
| Street address |
101 N Merion Ave
|
| City |
Bryn Mawr |
| State/province |
PA |
| ZIP/Postal code |
19096 |
| Country |
USA |
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| Platforms (1) |
| GPL17012 |
Illumina HiSeq 2000 (Streptomyces coelicolor) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA246145 |
| SRA |
SRP041654 |
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