This study aimed to investigate the effects of oral administration of lactic acid bacteria (LAB) on gene expression in murine ileum. To evaluate the strain specific and dose effects of LAB on the gene expression of distal ileum, Lactococcus lactis C59 and Lactobacillus rhamnosus GG were administered at a dose of 1 x 107 cells (10^7), 1 x 108 (10^8) cells or 1 x 109 (10^9) cells Microarray analysis was performed using total RNA from distal ileum to detail the gene expression of 7 groups; control, C59- and GG-administered at a dose of 1 x 107 cells (10^7), 1 x 108 (10^8) cells or 1 x 109 (10^9) cells. Gene expression patterns were clustered by administered strains. Several genes involved in immune system are up-regulated in the lower intestine in the presence of GG and down-regulated in the presence of C59.
Overall design
Balb/c mice (male, 7 week-old, n=6) in each group received 0.2ml (1 x 109 cells) of strain C59 or strain GG suspension for 2 weeks.
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