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| Status |
Public on Oct 03, 2018 |
| Title |
Global transcriptional analysis of Campylobacter jejuni NCTC 11168 after inactivation of flagella biosynthesis regulator FlhF |
| Organism |
Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819 |
| Experiment type |
Expression profiling by array
|
| Summary |
Campylobacter jejuni produces a single flagellum on one or both poles of the cell, the polar flagella not only confer the bacterium darting motility but also involved in its virulence and metabolic processes. FlhF is a lately defined flagella biosynthesis regulator, but how it regulates flagella assembly isn’t clear at present. Previous research had indicated FlhF was associated with gene expression, to understanding its role in flagella system and its correlation with C. jejuni virulence, the global gene expression profiles of C. jejuni NCTC 11168 after inactivation of FlhF was analyzed. Transcriptome analysis revealed that ten genes were significantly differentially expressed with 6 down-regulated and 4 up-regulated, these genes are involved in diverse cellular functions including bacterial chemotaxis, flagellar assembly and two-component system, significance analysis revealed that the pathway of flagellar assembly was remarkable affected. Further studies were focused on the flagella system, FlhF was found play a global role as 63% (41/65) of the flagella genes were down-regulated, it influences flagella gene transcription from the early stage, this effect persists during the synthesis process and has a remarkable impact on the late part. The results above expand our cognition of this regulator, and also helpful for comprehensively and systematically reveal its role in flagella system.
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| Overall design |
Gene expression profiles of C. jejuni strains were measured after grown in MH broth for 20h. Three biological replicates for broth C. jejuni NCTC 11168 (WT) and flhF mutant.
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| Contributor(s) |
Ren F, Wang N, Huang J, Jiao X |
| Citation(s) |
29458860, 30348090 |
| Submission date |
Oct 11, 2016 |
| Last update date |
Oct 29, 2018 |
| Contact name |
Fangzhe Ren |
| E-mail(s) |
rfz1989@126.com
|
| Organization name |
Yangzhou university
|
| Lab |
Jiangsu Key Lab of Zoonosis
|
| Street address |
East Wenhui Road No.12
|
| City |
Yangzhou |
| State/province |
Jiangsu |
| ZIP/Postal code |
225009 |
| Country |
China |
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| Platforms (1) |
| GPL22549 |
Agilent-061768 Campylobacter jejuni microarray (Probe name version) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA347860 |
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