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| Status |
Public on Mar 12, 2005 |
| Title |
MRS pH 5.5 (lactate)-2 (dye swap) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
L. acidophilus NCK1398 (NCFM::lacL)
|
| Organism |
Lactobacillus acidophilus |
| Extracted molecule |
total RNA |
| |
|
| Channel 2 |
| Source name |
L. acidophilus NCK1686 (NCFM::HPK)
|
| Organism |
Lactobacillus acidophilus |
| Extracted molecule |
total RNA |
| |
|
| |
| Description |
Early log phase cultures wt (NCK1398, control, Cy5) vs. test (mutant, NCK1686, Cy3) were exposed to MRS pH 5.5 (adjusted with lactate) for 30 min.
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| |
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| Submission date |
Nov 12, 2004 |
| Last update date |
May 27, 2005 |
| Contact name |
Todd Robert Klaenhammer |
| E-mail(s) |
trk@ncsu.edu, yjgoh@ncsu.edu, sjoflahe@ncsu.edu
|
| Phone |
919-515-2972
|
| Fax |
919 513 0014
|
| Organization name |
North Carolina State University
|
| Department |
Department of Food, Bioprocessing, and Nutrition Sciences
|
| Street address |
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27695 |
| Country |
USA |
| |
|
| Platform ID |
GPL1401 |
| Series (1) |
| GSE1976 |
Acid sensitivity and opp transport regulation in L. acidophilus |
|
| Data table header descriptions |
| ID_REF |
|
| RATIO |
(Cy5 Normalized - Background) / (Cy3 Normalized -Background) |
| VALUE |
log ratio (log2 of RATIO) |
| CH1_RAW |
raw signal output for channel 1 |
| CH1_BKD |
background signal output for channel 1 |
| CH1_SD |
standard deviation calculated for the mean signal for channel 1 |
| CH1_BKD_SD |
standard deviation for the mean background signal for channel 1 |
| CH2_RAW |
raw signal output for channel 2 |
| CH2_BKD |
background signal output for channel 2 |
| CH2_SD |
standard deviation calculated for the mean signal for channel 2 |
| CH2_BKD_SD |
standard deviation for the mean background signal for channel 2 |
| Data table |
| ID_REF |
RATIO |
VALUE |
CH1_RAW |
CH1_BKD |
CH1_SD |
CH1_BKD_SD |
CH2_RAW |
CH2_BKD |
CH2_SD |
CH2_BKD_SD |
| 1 |
1.368249657 |
0.452331495 |
2133.518555 |
105.666664 |
9087.572266 |
126.570198 |
4431.100586 |
229.083328 |
12782.0791 |
249.568634 |
| 2 |
1.290835053 |
0.36830466 |
460.97522 |
187.4375 |
530.160278 |
347.177002 |
824.387756 |
289.645844 |
403.794556 |
364.714081 |
| 3 |
1.818493285 |
0.862743599 |
479.427307 |
84.208336 |
412.491852 |
122.066246 |
1294.755615 |
206.3125 |
936.050476 |
172.84407 |
| 4 |
1.078296417 |
0.108753821 |
1817.726807 |
365.333344 |
1013.498108 |
277.795135 |
2779.553467 |
407.75 |
1796.462036 |
270.604889 |
| 5 |
1.486159848 |
0.571589298 |
1384.427368 |
458.041656 |
568.375 |
276.002472 |
2636.619629 |
551.583313 |
1540.709839 |
328.397919 |
| 6 |
1.120696363 |
0.164395453 |
2303.696533 |
576.583313 |
1241.071777 |
552.643188 |
3675.248779 |
743.916687 |
2252.596924 |
416.801331 |
| 7 |
0.992339112 |
-0.011094878 |
1519.22168 |
290.354156 |
963.738403 |
210.069641 |
2212.681641 |
365.875 |
1522.861572 |
235.784302 |
| 8 |
1.098152738 |
0.135078728 |
1425.009766 |
332.229156 |
820.933899 |
371.546631 |
2129.510254 |
312.104156 |
1414.481934 |
224.40979 |
| 9 |
1.10977921 |
0.150272681 |
1872.764648 |
486.270844 |
1082.819092 |
402.341736 |
2824.648438 |
494.354156 |
1798.682129 |
345.746948 |
| 10 |
1.210954194 |
0.276144294 |
1037.930054 |
142 |
1030.374268 |
192.973373 |
1846.181763 |
203.104172 |
1918.144775 |
172.817978 |
| 11 |
1.067785167 |
0.094621413 |
1075.641479 |
155.291672 |
968.081909 |
192.79335 |
1708.433838 |
220.125 |
1542.866699 |
215.29451 |
| 12 |
0.845977384 |
-0.241309 |
1114.180664 |
169.5625 |
810.915894 |
174.276855 |
1395.322754 |
185.083328 |
1519.655273 |
262.809692 |
| 13 |
1.032465092 |
0.046093005 |
2078.003906 |
338.625 |
1446.987061 |
243.692566 |
3138.730225 |
419 |
2128.392578 |
246.935608 |
| 14 |
1.061238422 |
0.085748814 |
2152.218506 |
353.958344 |
1278.041748 |
290.406677 |
3233.388184 |
343.229156 |
2017.126099 |
200.543121 |
| 15 |
1.221389551 |
0.288523408 |
2300.305176 |
375.375 |
1458.839111 |
284.829376 |
4035.680908 |
475.0625 |
2432.693115 |
313.658936 |
| 16 |
0.984777432 |
-0.022130395 |
4216.972656 |
152.083328 |
6107.529297 |
160.417252 |
6291.29541 |
228.916672 |
8897.417969 |
230.90477 |
| 17 |
0.921292204 |
-0.11826929 |
4482.408203 |
99.229164 |
5896.086914 |
121.968643 |
6340.27832 |
224.625 |
7923.491699 |
180.919952 |
| 18 |
1.239295956 |
0.309520758 |
2868.110352 |
114.458336 |
3885.779297 |
150.63327 |
5447.191406 |
278.979156 |
7184.522461 |
263.874359 |
| 19 |
0.714923101 |
-0.484140024 |
23339.12695 |
377.6875 |
17992.73828 |
252.898621 |
25299.38086 |
438.604156 |
19408.56055 |
291.851013 |
| 20 |
0.78196044 |
-0.354832473 |
24082.54688 |
330.979156 |
17860.52734 |
216.730392 |
28435.09961 |
307.458344 |
19632.24805 |
217.18959 |
Total number of rows: 6906
Table truncated, full table size 779 Kbytes.
| Supplementary data files not provided |
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