GEO DataSets

(Submitter supplied) In this work, we analyzed recognized PAM sequences from four CRISPR-Cas systems: E. coli I-E, B. halodurans I-C, S. thermophilus CR1 II-A, and F. novicida V. Cells containing functional PAMs were sorted using FACS and subsequently sequenced.

Organism:

Escherichia coli; Halalkalibacterium halodurans; Francisella tularensis subsp. novicida; Streptococcus thermophilus

Type:

Other

4 related Platforms

14 Samples

Download data: XLSX

(Submitter supplied) We report non-canonical PAM sequences for SpyCas9 that were revealed from a novel growth-based PAM screen in E. coli.

Organism:

Homo sapiens; Escherichia coli

Type:

Other

Platforms:

GPL15520 GPL16085

16 Samples

Download data: TXT

(Submitter supplied) We report the PAMs of phylogenetically-diverse Cas12a nucleases using a cell-free TXTL-based PAM screen. By adding a 5N randomized PAM library and Cas12a-gRNA in vitro, recognized PAM sequences were cleaved, while non-recognized PAMs remained. By amplifying the non-cleaved DNA, we used next-generation sequencing to analyze the depletion of functional PAMs of these Cas12a nucleases.

Organism:

synthetic construct

Type:

Other

Platform:

GPL17769

12 Samples

Download data: CSV

(Submitter supplied) We report the PAMs of diverse type I-E CRISPR- Cas systems and the type I-C and the type I-F1 CRISPR-Cas systems from Xanthomonas albilineans. Furthermore, we report PAMs of two type I-B CRISPR transposons (CASTs) and the Vibrio cholerae type I-F CAST. For identification of the PAMs, we used a cell-free TXTL-based PAM screen we named PAM-DETECT. By adding a 5N randomized PAM library and plasmids encoding for Cascade genes and gRNAs, recognized PAMs were bound by Cascade and protected from cleavage by a restriction enzyme that has it's recognition site within the target region. more...

Organism:

synthetic construct; Escherichia coli

Type:

Other

Platforms:

GPL26526 GPL16085 GPL17769

89 Samples

Download data: TXT