|
| Status |
Public on Feb 17, 2016 |
| Title |
Deep sequencing data for PAM-SCANR tested CRISPR-Cas systems |
| Organisms |
Francisella tularensis subsp. novicida; Escherichia coli; Streptococcus thermophilus; Halalkalibacterium halodurans |
| Experiment type |
Other
|
| Summary |
In this work, we analyzed recognized PAM sequences from four CRISPR-Cas systems: E. coli I-E, B. halodurans I-C, S. thermophilus CR1 II-A, and F. novicida V. Cells containing functional PAMs were sorted using FACS and subsequently sequenced.
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| |
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| Overall design |
Ranking of recognized PAMs from a positive screen
|
| |
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| Contributor(s) |
Leenay RT, Maksimchuk KR, Slotkowski RA, Agrawal RA, Gomaa AA, Briner AE, Barrangou R, Beisel C |
| Citation(s) |
27041224 |
| Submission date |
Dec 04, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Chase Beisel |
| E-mail(s) |
cbeisel@ncsu.edu
|
| Phone |
9195158395
|
| Organization name |
North Carolina State University
|
| Department |
Chemical and Biolmolecular Engineering
|
| Street address |
840 Main Campus Drive, Partners II Rm 2109
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
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| Platforms (4)
|
| GPL16085 |
Illumina MiSeq (Escherichia coli) |
| GPL21209 |
Illumina MiSeq (Streptococcus thermophilus) |
| GPL21210 |
Illumina MiSeq (Francisella tularensis subsp. novicida) |
| GPL21211 |
Illumina MiSeq (Bacillus halodurans) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA305147 |
| SRA |
SRP067010 |
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