|
| Status |
Public on May 23, 2007 |
| Title |
Technical Microbiology-Viable beer spoilage bacteria microarray V2 |
| Technology type |
spotted oligonucleotide |
| Distribution |
non-commercial |
| Organisms |
Pectinatus frisingensis; Levilactobacillus brevis; Lacticaseibacillus casei; Loigolactobacillus coryniformis; Megasphaera cerevisiae; Pediococcus damnosus; Pectinatus cerevisiiphilus; Schleiferilactobacillus perolens; Pediococcus inopinatus |
| Manufacturer |
Institut für Biotechnologie 1 |
| Manufacture protocol |
Standard glass microscope slides were coated with a poly-L-lysine solution and stored for several weeks to allow the surface to become sufficiently hydrophobic. Species-specific probes were spotted in 3x SSC onto the glass surface using a robotic printing system. For routine experiments 50 μM was used as probe concentrations. The diameter of the spots was 150 μm and the space between the centres of two spots was 205 μm allowing the preparation of seven grids each containing 70 spot replicates. To distribute the oligonucleotides more evenly, the spots were rehydrated using a 1x SSC bath at 40°C until the spots were glistening, followed by snapped-drying at 120°C using a heat block. To increase the amount of hybridisable oligonucleotides, stably attached to each spot, the nucleic acids were cross-linked by UV irradiation.
|
| Support |
glass |
| Coating |
polysine |
| |
|
| Description |
Each oligonucleotide species-specific probe was spotted in 70 replicate spots on the microarray. Nine single beer spoilage bacteria species were detected with the developed prototype oligonucleotide microarray and also three mixes of two different beer spoilage bacteria species.
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| |
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| Contributor(s) |
Weber DG, Sahm K, Polen T, Wendisch VF, Antranikian G |
| Submission date |
May 11, 2007 |
| Last update date |
May 23, 2007 |
| Contact name |
Daniel Gilbert Weber |
| Organization name |
IPA
|
| Department |
Molekulare Medizin
|
| Street address |
Bürkle-de-la-Camp Platz 1
|
| City |
Bochum |
| ZIP/Postal code |
44789 |
| Country |
Germany |
| |
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| Samples (4) |
GSM190246, GSM190247, GSM190248, GSM190249 |
| Series (1) |
| GSE7840 |
Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria |
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