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| Status |
Public on May 23, 2007 |
| Title |
Oligonucleotide microarrays for the detection and identification of viable beer spoilage bacteria |
| Organisms |
Pectinatus frisingensis; Levilactobacillus brevis; Lacticaseibacillus casei; Loigolactobacillus coryniformis; Megasphaera cerevisiae; Pediococcus damnosus; Pectinatus cerevisiiphilus; Schleiferilactobacillus perolens; Pediococcus inopinatus |
| Experiment type |
Expression profiling by array
|
| Summary |
A prototype oligonucleotide microarray was designed to detect and identify viable bacterial species with the potential to grow of common beer spoilage microorganisms from the genera Lactobacillus, Megasphaera, Pediococcus and Pectinatus. Probes targeted the intergenic spacer regions (ISR) between 16S and 23S rRNA, which were amplified in a combination of reverse transcriptase (RT) and polymerase chain reaction (PCR) prior to hybridization. This method allows the detection and discrimination of single bacterial species in a complex sample. Furthermore, microarrays using oligonucleotide probes targeting the ISR allow the distinction between viable bacteria with the potential to grow and non-growing bacteria. The results demonstrate the feasibility of oligonucleotide microarrays as a contamination control in food industry for the detection and identification of spoilage microorganisms within mixed population.
Keywords: microarray, oligonucleotide, species-specific, detection, beer spoilage bacteria
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| Overall design |
Each oligonucleotide species-specific probe was spotted in 70 replicate spots on the microarray. Nine single beer spoilage bacteria species were detected with the developed prototype oligonucleotide microarray and also three mixes of two different beer spoilage bacteria species.
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| Contributor(s) |
Weber DG, Sahm K, Polen T, Wendisch VF, Antranikian G |
| Citation(s) |
18785882 |
| Submission date |
May 18, 2007 |
| Last update date |
Apr 14, 2017 |
| Contact name |
Daniel Gilbert Weber |
| Organization name |
IPA
|
| Department |
Molekulare Medizin
|
| Street address |
Bürkle-de-la-Camp Platz 1
|
| City |
Bochum |
| ZIP/Postal code |
44789 |
| Country |
Germany |
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| Platforms (2) |
| GPL5157 |
Technical Microbiology-Viable beer spoilage bacteria microarray V1 |
| GPL5158 |
Technical Microbiology-Viable beer spoilage bacteria microarray V2 |
|
| Samples (12)
|
| GSM190233 |
Lactobacillus casei detected by microarray |
| GSM190235 |
Lactobacillus coryniiformis detected by microarray |
| GSM190236 |
Lactobacillus perolens detected by microarray |
| GSM190237 |
Pediococcud damnosus detected by microarray |
| GSM190239 |
Pediococcus inopinatus detected by microarray |
| GSM190240 |
Megasphaera cerevisiae detected by microarray |
| GSM190242 |
Pectinatus cerevisiiphilus detected by microarray |
| GSM190244 |
Megasphaera cerevisiae/Pectinatus cerevisiiphilus mix detected by microarray |
| GSM190246 |
Lactobacillus brevis detected by microarray |
| GSM190247 |
Pectinatus frisingensis detected by microarray |
| GSM190248 |
Lactobacillus brevis and Pectinatus frisingensis detected by microarray |
| GSM190249 |
Pediococcus inopinatus and Pectinatus cerevisiiphilus detected by microarray |
|
| Relations |
| BioProject |
PRJNA99965 |
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